Cargando…
The Protective Effects of Lycium Barbarum Polysaccharides on Transient Retinal Ischemia
Retinal ischemia/reperfusion (I/R) injury leads to irreversible neuronal death, glial activation, retinal swelling and oxidative stress. It is a common feature in various ocular diseases, such as glaucoma, diabetic retinopathy and amaurosis fugax. In the present study, we aimed to evaluate the effec...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2011
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393857/ http://dx.doi.org/10.1068/ic301 |
_version_ | 1783229648328458240 |
---|---|
author | Yang, Di Li, Suk-Yee Yeung, Chung-Man Yu, Wing-Yan Chang, Raymond Chuen-Chung So, Kwok-Fai Wong, David Lo, Amy C. Y. |
author_facet | Yang, Di Li, Suk-Yee Yeung, Chung-Man Yu, Wing-Yan Chang, Raymond Chuen-Chung So, Kwok-Fai Wong, David Lo, Amy C. Y. |
author_sort | Yang, Di |
collection | PubMed |
description | Retinal ischemia/reperfusion (I/R) injury leads to irreversible neuronal death, glial activation, retinal swelling and oxidative stress. It is a common feature in various ocular diseases, such as glaucoma, diabetic retinopathy and amaurosis fugax. In the present study, we aimed to evaluate the effects of Lycium Barbarum Polysaccharides (LBP) in a murine retinal I/R model. Mice were orally treated with either vehicle (PBS) or LBP (1mg/kg) daily for 1 week before induction of retinal ischemia. Retinae were collected after 2 hours ischemia and 22 hours reperfusion. Paraffin-embedded sections were prepared for immunohistochemical analyses. Significantly fewer viable cells were found in vehicle-treated retinae comparing to LBP group. This finding was further confirmed by TUNEL assay where significantly fewer apoptotic cells were identified in LBP-treated retinae. Additionally, retinal swelling induced by retinal I/R injury in the vehicle-treated group was not observed in LBP-treated group. Moreover, intense GFAP immunoreactivity and IgG extravasation were observed in vehicle-treated group but not in LBP treated group. The results showed that pre-treatment with LBP was protective in retinal I/R injury via reducing neuronal death, apoptosis, retinal swelling, GFAP activation and blood vessel leakage. LBP may be used as a preventive agent for retinal ischemia diseases. |
format | Online Article Text |
id | pubmed-5393857 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-53938572017-04-24 The Protective Effects of Lycium Barbarum Polysaccharides on Transient Retinal Ischemia Yang, Di Li, Suk-Yee Yeung, Chung-Man Yu, Wing-Yan Chang, Raymond Chuen-Chung So, Kwok-Fai Wong, David Lo, Amy C. Y. Iperception Article Retinal ischemia/reperfusion (I/R) injury leads to irreversible neuronal death, glial activation, retinal swelling and oxidative stress. It is a common feature in various ocular diseases, such as glaucoma, diabetic retinopathy and amaurosis fugax. In the present study, we aimed to evaluate the effects of Lycium Barbarum Polysaccharides (LBP) in a murine retinal I/R model. Mice were orally treated with either vehicle (PBS) or LBP (1mg/kg) daily for 1 week before induction of retinal ischemia. Retinae were collected after 2 hours ischemia and 22 hours reperfusion. Paraffin-embedded sections were prepared for immunohistochemical analyses. Significantly fewer viable cells were found in vehicle-treated retinae comparing to LBP group. This finding was further confirmed by TUNEL assay where significantly fewer apoptotic cells were identified in LBP-treated retinae. Additionally, retinal swelling induced by retinal I/R injury in the vehicle-treated group was not observed in LBP-treated group. Moreover, intense GFAP immunoreactivity and IgG extravasation were observed in vehicle-treated group but not in LBP treated group. The results showed that pre-treatment with LBP was protective in retinal I/R injury via reducing neuronal death, apoptosis, retinal swelling, GFAP activation and blood vessel leakage. LBP may be used as a preventive agent for retinal ischemia diseases. SAGE Publications 2011-05-01 2011-05 /pmc/articles/PMC5393857/ http://dx.doi.org/10.1068/ic301 Text en © 2011 SAGE Publications Ltd. Manuscript content on this site is licensed under Creative Commons Licenses http://creativecommons.org/licenses/by-nc-nd/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 License (http://www.creativecommons.org/licenses/by-nc-nd/3.0/) which permits non-commercial use, reproduction and distribution of the work as published without adaptation or alteration, without further permission provided the original work is attributed as specified on the SAGE and Open Access page (http://www.uk.sagepub.com/aboutus/openaccess.htm). |
spellingShingle | Article Yang, Di Li, Suk-Yee Yeung, Chung-Man Yu, Wing-Yan Chang, Raymond Chuen-Chung So, Kwok-Fai Wong, David Lo, Amy C. Y. The Protective Effects of Lycium Barbarum Polysaccharides on Transient Retinal Ischemia |
title | The Protective Effects of Lycium Barbarum Polysaccharides on Transient Retinal Ischemia |
title_full | The Protective Effects of Lycium Barbarum Polysaccharides on Transient Retinal Ischemia |
title_fullStr | The Protective Effects of Lycium Barbarum Polysaccharides on Transient Retinal Ischemia |
title_full_unstemmed | The Protective Effects of Lycium Barbarum Polysaccharides on Transient Retinal Ischemia |
title_short | The Protective Effects of Lycium Barbarum Polysaccharides on Transient Retinal Ischemia |
title_sort | protective effects of lycium barbarum polysaccharides on transient retinal ischemia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5393857/ http://dx.doi.org/10.1068/ic301 |
work_keys_str_mv | AT yangdi theprotectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT lisukyee theprotectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT yeungchungman theprotectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT yuwingyan theprotectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT changraymondchuenchung theprotectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT sokwokfai theprotectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT wongdavid theprotectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT loamycy theprotectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT yangdi protectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT lisukyee protectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT yeungchungman protectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT yuwingyan protectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT changraymondchuenchung protectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT sokwokfai protectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT wongdavid protectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia AT loamycy protectiveeffectsoflyciumbarbarumpolysaccharidesontransientretinalischemia |