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The ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, Schistocerca gregaria

Insects are enclosed in a rigid exoskeleton, providing protection from desiccation and mechanical injury. To allow growth, this armour needs to be replaced regularly in a process called moulting. Moulting entails the production of a new exoskeleton and shedding of the old one and is induced by a pul...

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Autores principales: Lenaerts, Cynthia, Cools, Dorien, Verdonck, Rik, Verbakel, Lina, Vanden Broeck, Jozef, Marchal, Elisabeth
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394484/
https://www.ncbi.nlm.nih.gov/pubmed/28417966
http://dx.doi.org/10.1038/srep46502
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author Lenaerts, Cynthia
Cools, Dorien
Verdonck, Rik
Verbakel, Lina
Vanden Broeck, Jozef
Marchal, Elisabeth
author_facet Lenaerts, Cynthia
Cools, Dorien
Verdonck, Rik
Verbakel, Lina
Vanden Broeck, Jozef
Marchal, Elisabeth
author_sort Lenaerts, Cynthia
collection PubMed
description Insects are enclosed in a rigid exoskeleton, providing protection from desiccation and mechanical injury. To allow growth, this armour needs to be replaced regularly in a process called moulting. Moulting entails the production of a new exoskeleton and shedding of the old one and is induced by a pulse in ecdysteroids, which activates a peptide-mediated signalling cascade. In Holometabola, ecdysis triggering hormone (ETH) is the key factor in this cascade. Very little functional information is available in Hemimetabola, which display a different kind of development characterized by gradual changes. This paper reports on the identification of the ETH precursor and the pharmacological and functional characterisation of the ETH receptor in a hemimetabolous pest species, the desert locust, Schistocerca gregaria. Activation of SchgrETHR by SchgrETH results in an increase of both Ca(2+) and cyclic AMP, suggesting that SchgrETHR displays dual coupling properties in an in vitro cell-based assay. Using qRT-PCR, an in-depth profiling study of SchgrETH and SchgrETHR transcripts was performed. Silencing of SchgrETH and SchgrETHR resulted in lethality at the expected time of ecdysis, thereby showing their crucial role in moulting.
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spelling pubmed-53944842017-04-20 The ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, Schistocerca gregaria Lenaerts, Cynthia Cools, Dorien Verdonck, Rik Verbakel, Lina Vanden Broeck, Jozef Marchal, Elisabeth Sci Rep Article Insects are enclosed in a rigid exoskeleton, providing protection from desiccation and mechanical injury. To allow growth, this armour needs to be replaced regularly in a process called moulting. Moulting entails the production of a new exoskeleton and shedding of the old one and is induced by a pulse in ecdysteroids, which activates a peptide-mediated signalling cascade. In Holometabola, ecdysis triggering hormone (ETH) is the key factor in this cascade. Very little functional information is available in Hemimetabola, which display a different kind of development characterized by gradual changes. This paper reports on the identification of the ETH precursor and the pharmacological and functional characterisation of the ETH receptor in a hemimetabolous pest species, the desert locust, Schistocerca gregaria. Activation of SchgrETHR by SchgrETH results in an increase of both Ca(2+) and cyclic AMP, suggesting that SchgrETHR displays dual coupling properties in an in vitro cell-based assay. Using qRT-PCR, an in-depth profiling study of SchgrETH and SchgrETHR transcripts was performed. Silencing of SchgrETH and SchgrETHR resulted in lethality at the expected time of ecdysis, thereby showing their crucial role in moulting. Nature Publishing Group 2017-04-18 /pmc/articles/PMC5394484/ /pubmed/28417966 http://dx.doi.org/10.1038/srep46502 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Lenaerts, Cynthia
Cools, Dorien
Verdonck, Rik
Verbakel, Lina
Vanden Broeck, Jozef
Marchal, Elisabeth
The ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, Schistocerca gregaria
title The ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, Schistocerca gregaria
title_full The ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, Schistocerca gregaria
title_fullStr The ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, Schistocerca gregaria
title_full_unstemmed The ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, Schistocerca gregaria
title_short The ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, Schistocerca gregaria
title_sort ecdysis triggering hormone system is essential for successful moulting of a major hemimetabolous pest insect, schistocerca gregaria
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394484/
https://www.ncbi.nlm.nih.gov/pubmed/28417966
http://dx.doi.org/10.1038/srep46502
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