3D correlative light and electron microscopy of cultured cells using serial blockface scanning electron microscopy

The processes of life take place in multiple dimensions, but imaging these processes in even three dimensions is challenging. Here, we describe a workflow for 3D correlative light and electron microscopy (CLEM) of cell monolayers using fluorescence microscopy to identify and follow biological events...

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Detalles Bibliográficos
Autores principales: Russell, Matthew R. G., Lerner, Thomas R., Burden, Jemima J., Nkwe, David O., Pelchen-Matthews, Annegret, Domart, Marie-Charlotte, Durgan, Joanne, Weston, Anne, Jones, Martin L., Peddie, Christopher J., Carzaniga, Raffaella, Florey, Oliver, Marsh, Mark, Gutierrez, Maximiliano G., Collinson, Lucy M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Tools and Techniques
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394779/
https://www.ncbi.nlm.nih.gov/pubmed/27445312
http://dx.doi.org/10.1242/jcs.188433

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