A Comparison of Molecular Typing Methods Applied to Enterobacter cloacae complex: hsp60 Sequencing, Rep-PCR, and MLST

Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak...

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Detalles Bibliográficos
Autores principales: Viau, Roberto A., Kiedrowski, Lee M., Kreiswirth, Barry N., Adams, Mark, Perez, Federico, Marchaim, Dror, Guerrero, Dubert M., Kaye, Keith S., Logan, Latania K., Villegas, Maria Virginia, Bonomo, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pathogens and Immunity 2017
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5394936/
https://www.ncbi.nlm.nih.gov/pubmed/28428984
http://dx.doi.org/10.20411/pai.v2i1.99
Descripción
Sumario:Molecular typing using repetitive sequenced-based PCR (rep-PCR) and hsp60 sequencing were applied to a collection of diverse Enterobacter cloacae complex isolates. To determine the most practical method for reference laboratories, we analyzed 71 E. cloacae complex isolates from sporadic and outbreak occurrences originating from 4 geographic areas. While rep-PCR was more discriminating, hsp60 sequencing provided a broader and a more objective geographical tracking method similar to multilocus sequence typing (MLST). In addition, we suggest that MLST may have higher discriminative power compared to hsp60 sequencing, although rep-PCR remains the most discriminative method for local outbreak investigations. In addition, rep-PCR can be an effective and inexpensive method for local outbreak investigation.

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