Cargando…

Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran

Local markets provide a rapid insight into the medicinal plants growing in a region as well as local traditional health concerns. Identification of market plant material can be challenging as plants are often sold in dried or processed forms. In this study, three approaches of DNA barcoding-based mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Ghorbani, Abdolbaset, Saeedi, Yousef, de Boer, Hugo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395179/
https://www.ncbi.nlm.nih.gov/pubmed/28419161
http://dx.doi.org/10.1371/journal.pone.0175722
_version_ 1783229831612203008
author Ghorbani, Abdolbaset
Saeedi, Yousef
de Boer, Hugo J.
author_facet Ghorbani, Abdolbaset
Saeedi, Yousef
de Boer, Hugo J.
author_sort Ghorbani, Abdolbaset
collection PubMed
description Local markets provide a rapid insight into the medicinal plants growing in a region as well as local traditional health concerns. Identification of market plant material can be challenging as plants are often sold in dried or processed forms. In this study, three approaches of DNA barcoding-based molecular identification of market samples are evaluated, two objective sequence matching approaches and an integrative approach that coalesces sequence matching with a priori and a posteriori data from other markers, morphology, ethnoclassification and species distribution. Plant samples from markets and herbal shops were identified using morphology, descriptions of local use, and vernacular names with relevant floras and pharmacopoeias. DNA barcoding was used for identification of samples that could not be identified to species level using morphology. Two methods based on BLAST similarity-based identification, were compared with an integrative identification approach. Integrative identification combining the optimized similarity-based approach with a priori and a posteriori information resulted in a 1.67, 1.95 and 2.00 fold increase for ITS, trnL-F spacer, and both combined, respectively. DNA barcoding of traded plant material requires objective strategies to include data from multiple markers, morphology, and traditional knowledge to optimize species level identification success.
format Online
Article
Text
id pubmed-5395179
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-53951792017-05-04 Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran Ghorbani, Abdolbaset Saeedi, Yousef de Boer, Hugo J. PLoS One Research Article Local markets provide a rapid insight into the medicinal plants growing in a region as well as local traditional health concerns. Identification of market plant material can be challenging as plants are often sold in dried or processed forms. In this study, three approaches of DNA barcoding-based molecular identification of market samples are evaluated, two objective sequence matching approaches and an integrative approach that coalesces sequence matching with a priori and a posteriori data from other markers, morphology, ethnoclassification and species distribution. Plant samples from markets and herbal shops were identified using morphology, descriptions of local use, and vernacular names with relevant floras and pharmacopoeias. DNA barcoding was used for identification of samples that could not be identified to species level using morphology. Two methods based on BLAST similarity-based identification, were compared with an integrative identification approach. Integrative identification combining the optimized similarity-based approach with a priori and a posteriori information resulted in a 1.67, 1.95 and 2.00 fold increase for ITS, trnL-F spacer, and both combined, respectively. DNA barcoding of traded plant material requires objective strategies to include data from multiple markers, morphology, and traditional knowledge to optimize species level identification success. Public Library of Science 2017-04-18 /pmc/articles/PMC5395179/ /pubmed/28419161 http://dx.doi.org/10.1371/journal.pone.0175722 Text en © 2017 Ghorbani et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ghorbani, Abdolbaset
Saeedi, Yousef
de Boer, Hugo J.
Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran
title Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran
title_full Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran
title_fullStr Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran
title_full_unstemmed Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran
title_short Unidentifiable by morphology: DNA barcoding of plant material in local markets in Iran
title_sort unidentifiable by morphology: dna barcoding of plant material in local markets in iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395179/
https://www.ncbi.nlm.nih.gov/pubmed/28419161
http://dx.doi.org/10.1371/journal.pone.0175722
work_keys_str_mv AT ghorbaniabdolbaset unidentifiablebymorphologydnabarcodingofplantmaterialinlocalmarketsiniran
AT saeediyousef unidentifiablebymorphologydnabarcodingofplantmaterialinlocalmarketsiniran
AT deboerhugoj unidentifiablebymorphologydnabarcodingofplantmaterialinlocalmarketsiniran