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Multispot single-molecule FRET: High-throughput analysis of freely diffusing molecules

We describe an 8-spot confocal setup for high-throughput smFRET assays and illustrate its performance with two characteristic experiments. First, measurements on a series of freely diffusing doubly-labeled dsDNA samples allow us to demonstrate that data acquired in multiple spots in parallel can be...

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Detalles Bibliográficos
Autores principales:	Ingargiola, Antonino, Lerner, Eitan, Chung, SangYoon, Panzeri, Francesco, Gulinatti, Angelo, Rech, Ivan, Ghioni, Massimo, Weiss, Shimon, Michalet, Xavier
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2017
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395192/ https://www.ncbi.nlm.nih.gov/pubmed/28419142 http://dx.doi.org/10.1371/journal.pone.0175766

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