Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain

Penicillin-binding proteins (PBPs) are responsible for peptidoglycan synthesis. By using biotinylated ampicillin, we detected PBPs of Lactobacillus paracasei strains. Ten PBPs were identified, 7 of which had apparent molecular sizes similar to those of Escherichia coli. In the presence of cholate, s...

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Autores principales: HATTORI, Masahiro, TORRES, Glaezel Angelique, TANAKA, Naoto, OKADA, Sanae, ENDO, Akihito, NAKAGAWA, Junichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BMFH Press 2016
Materias:
Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395426/
https://www.ncbi.nlm.nih.gov/pubmed/28439489
http://dx.doi.org/10.12938/bmfh.16-019
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author HATTORI, Masahiro
TORRES, Glaezel Angelique
TANAKA, Naoto
OKADA, Sanae
ENDO, Akihito
NAKAGAWA, Junichi
author_facet HATTORI, Masahiro
TORRES, Glaezel Angelique
TANAKA, Naoto
OKADA, Sanae
ENDO, Akihito
NAKAGAWA, Junichi
author_sort HATTORI, Masahiro
collection PubMed
description Penicillin-binding proteins (PBPs) are responsible for peptidoglycan synthesis. By using biotinylated ampicillin, we detected PBPs of Lactobacillus paracasei strains. Ten PBPs were identified, 7 of which had apparent molecular sizes similar to those of Escherichia coli. In the presence of cholate, strain NRIC 0625 showed an elongated shape, and its putative PBP3 showed cholate-sensitive penicillin-binding activity. Furthermore, this strain was highly sensitive to cefalexin, which is known to inhibit cell division by inactivating PBP3. These results suggest that the septum synthetase PBP3 of lactic acid bacteria can be one of the targets of intestinal bile acid.
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spelling pubmed-53954262017-04-24 Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain HATTORI, Masahiro TORRES, Glaezel Angelique TANAKA, Naoto OKADA, Sanae ENDO, Akihito NAKAGAWA, Junichi Biosci Microbiota Food Health Note Penicillin-binding proteins (PBPs) are responsible for peptidoglycan synthesis. By using biotinylated ampicillin, we detected PBPs of Lactobacillus paracasei strains. Ten PBPs were identified, 7 of which had apparent molecular sizes similar to those of Escherichia coli. In the presence of cholate, strain NRIC 0625 showed an elongated shape, and its putative PBP3 showed cholate-sensitive penicillin-binding activity. Furthermore, this strain was highly sensitive to cefalexin, which is known to inhibit cell division by inactivating PBP3. These results suggest that the septum synthetase PBP3 of lactic acid bacteria can be one of the targets of intestinal bile acid. BMFH Press 2016-11-25 2017 /pmc/articles/PMC5395426/ /pubmed/28439489 http://dx.doi.org/10.12938/bmfh.16-019 Text en BMFH Press This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Note
HATTORI, Masahiro
TORRES, Glaezel Angelique
TANAKA, Naoto
OKADA, Sanae
ENDO, Akihito
NAKAGAWA, Junichi
Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain
title Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain
title_full Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain
title_fullStr Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain
title_full_unstemmed Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain
title_short Detection and analysis of Lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain
title_sort detection and analysis of lactobacillus paracasei penicillin-binding proteins revealed the presence of cholate-sensitive penicillin-binding protein 3 and an elongated cell shape in a cholate-sensitive strain
topic Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395426/
https://www.ncbi.nlm.nih.gov/pubmed/28439489
http://dx.doi.org/10.12938/bmfh.16-019
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