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Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth
Treatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) cont...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395512/ https://www.ncbi.nlm.nih.gov/pubmed/28443212 http://dx.doi.org/10.1186/s40064-016-2263-9 |
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author | Demirel, Sezin Yalvac, Mehmet Emir Tapsin, Sidika Akyuz, Serap Ak, Esin Cetinel, Sule Yarat, Aysen Sahin, Fikrettin |
author_facet | Demirel, Sezin Yalvac, Mehmet Emir Tapsin, Sidika Akyuz, Serap Ak, Esin Cetinel, Sule Yarat, Aysen Sahin, Fikrettin |
author_sort | Demirel, Sezin |
collection | PubMed |
description | Treatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) contains thrombin and fibrinogen to form an adhesive fibrin clot routinely used in surgical procedures. Here, we aimed to investigate the effects of ATSCs + FS treatment on healing of PDL after tooth replantation in a rat model. After 60 min of extraction, maxillary central incisor teeth were replanted with ATSCs + FS. Two months later, the rats were sacrificed and hemimaxilla blocks were dissected out for histological analysis. The results showed that there was a significant improvement in histological findings of ATSCs + FS treated group compared to only FS treated and non-treated groups corresponding to reduced inflammatory resorption and increased new PDL formation. Furthermore, the ankylosis levels were lowered after ATSCs + FS treatment. Singular use of FS improved PDL healing moderately. Our results indicated that ATSCs + FS treatment improves PDL healing after tooth replantation suggesting a new therapeutic potential in the treatment of dental avulsion cases. |
format | Online Article Text |
id | pubmed-5395512 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-53955122017-04-25 Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth Demirel, Sezin Yalvac, Mehmet Emir Tapsin, Sidika Akyuz, Serap Ak, Esin Cetinel, Sule Yarat, Aysen Sahin, Fikrettin Springerplus Research Treatment for dental avulsion cases is early or late replantation of the traumatized teeth. Prognosis of the replanted tooth depends on the level of periodontal injury. Adipose tissue stem cells (ATSCs) were reported to improve periodontal ligament tissue (PDL) regeneration. Fibrin sealant (FS) contains thrombin and fibrinogen to form an adhesive fibrin clot routinely used in surgical procedures. Here, we aimed to investigate the effects of ATSCs + FS treatment on healing of PDL after tooth replantation in a rat model. After 60 min of extraction, maxillary central incisor teeth were replanted with ATSCs + FS. Two months later, the rats were sacrificed and hemimaxilla blocks were dissected out for histological analysis. The results showed that there was a significant improvement in histological findings of ATSCs + FS treated group compared to only FS treated and non-treated groups corresponding to reduced inflammatory resorption and increased new PDL formation. Furthermore, the ankylosis levels were lowered after ATSCs + FS treatment. Singular use of FS improved PDL healing moderately. Our results indicated that ATSCs + FS treatment improves PDL healing after tooth replantation suggesting a new therapeutic potential in the treatment of dental avulsion cases. Springer International Publishing 2016-05-17 /pmc/articles/PMC5395512/ /pubmed/28443212 http://dx.doi.org/10.1186/s40064-016-2263-9 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Research Demirel, Sezin Yalvac, Mehmet Emir Tapsin, Sidika Akyuz, Serap Ak, Esin Cetinel, Sule Yarat, Aysen Sahin, Fikrettin Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth |
title | Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth |
title_full | Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth |
title_fullStr | Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth |
title_full_unstemmed | Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth |
title_short | Tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth |
title_sort | tooth replantation with adipose tissue stem cells and fibrin sealant: microscopic analysis of rat’s teeth |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395512/ https://www.ncbi.nlm.nih.gov/pubmed/28443212 http://dx.doi.org/10.1186/s40064-016-2263-9 |
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