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Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation
OBJECTIVE: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca(2)(+) chelator treatment at the time of fertilization on the transitio...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society for Reproductive Medicine
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395547/ https://www.ncbi.nlm.nih.gov/pubmed/28428939 http://dx.doi.org/10.5653/cerm.2017.44.1.15 |
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author | Yoon, Jeong Juhn, Kyoung-Mi Yoon, San-Hyun Ko, Yong Lim, Jin-Ho |
author_facet | Yoon, Jeong Juhn, Kyoung-Mi Yoon, San-Hyun Ko, Yong Lim, Jin-Ho |
author_sort | Yoon, Jeong |
collection | PubMed |
description | OBJECTIVE: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca(2)(+) chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). METHODS: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a Ca(2)(+) chelator to investigate the effect of Ca(2)(+) oscillations on their maturation. RESULTS: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the Ca(2)(+) chelator-treated group. CONCLUSION: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by Ca(2)(+) chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular Ca(2)(+) oscillations driven by fertilization. |
format | Online Article Text |
id | pubmed-5395547 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Korean Society for Reproductive Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-53955472017-04-20 Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation Yoon, Jeong Juhn, Kyoung-Mi Yoon, San-Hyun Ko, Yong Lim, Jin-Ho Clin Exp Reprod Med Original Article OBJECTIVE: The aims of this study were to investigate whether fertilization could induce the resumption of meiosis in mouse oocytes arrested at metaphase I (MI) after in vitro maturation (IVM), and to investigate the effect of Ca(2)(+) chelator treatment at the time of fertilization on the transition from MI to metaphase II (MII). METHODS: MII-stage and arrested MI-stage mouse oocytes after IVM were fertilized, and then embryonic development was monitored. Blastocysts from each group were transferred into 2.5 days post-coitum pseudo-pregnant ICR mice. MI oocytes after IVM were treated with a Ca(2)(+) chelator to investigate the effect of Ca(2)(+) oscillations on their maturation. RESULTS: As insemination time increased, the number of oocytes in the MI group that reached the MII stage also increased. The blastocyst rates and total cell numbers in the MII group were significantly higher than in the MI group. No pregnancy occurred in the MI group, but 10 pregnancies were achieved (10 of 12) in the MII group. The proportion of MI oocytes that matured to MII oocytes after fertilization was significantly higher in the non-treated group than in the Ca(2)(+) chelator-treated group. CONCLUSION: The findings that a higher proportion of MI-arrested oocytes progressed to MII after fertilization and that the MI-to-MII transition was blocked by Ca(2)(+) chelator treatments before fertilization indicate that the maturation of MI oocytes to MII oocytes is associated with intracellular Ca(2)(+) oscillations driven by fertilization. The Korean Society for Reproductive Medicine 2017-03 2017-03-31 /pmc/articles/PMC5395547/ /pubmed/28428939 http://dx.doi.org/10.5653/cerm.2017.44.1.15 Text en Copyright © 2017. The Korean Society for Reproductive Medicine http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Yoon, Jeong Juhn, Kyoung-Mi Yoon, San-Hyun Ko, Yong Lim, Jin-Ho Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation |
title | Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation |
title_full | Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation |
title_fullStr | Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation |
title_full_unstemmed | Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation |
title_short | Effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase I after in vitro maturation |
title_sort | effects of sperm insemination on the final meiotic maturation of mouse oocytes arrested at metaphase i after in vitro maturation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5395547/ https://www.ncbi.nlm.nih.gov/pubmed/28428939 http://dx.doi.org/10.5653/cerm.2017.44.1.15 |
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