Isolation and Characterization of an Aeromonas punctata Bacteriophage

An Aeromonas punctata bacteriophage, named as DH1, was isolated from East Lake, Wuhan city, China. Morphologically, phage DH1 showed a typical Myoviridae structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage had a latent period of about 90 minutes and an...

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Autores principales: Kai, Cheng, Denglan, Zhang, Jingxuan, Deng, Yijun, Zhao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Open 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396062/
https://www.ncbi.nlm.nih.gov/pubmed/28484577
http://dx.doi.org/10.2174/1874120701509010185
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author Kai, Cheng
Denglan, Zhang
Jingxuan, Deng
Yijun, Zhao
author_facet Kai, Cheng
Denglan, Zhang
Jingxuan, Deng
Yijun, Zhao
author_sort Kai, Cheng
collection PubMed
description An Aeromonas punctata bacteriophage, named as DH1, was isolated from East Lake, Wuhan city, China. Morphologically, phage DH1 showed a typical Myoviridae structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage had a latent period of about 90 minutes and an average burst size of about 125 PFU•Cell(-1). Restriction enzyme pattern of the bacteriophage’s genome showed that the genome is a double-stranded DNA and about 34kb in size. The sequenced genomic fragments showed highly similarities to gp04 and gp16 sequence of other Myoviridae bacteriophages at protein level.
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spelling pubmed-53960622017-05-08 Isolation and Characterization of an Aeromonas punctata Bacteriophage Kai, Cheng Denglan, Zhang Jingxuan, Deng Yijun, Zhao Open Biomed Eng J Article An Aeromonas punctata bacteriophage, named as DH1, was isolated from East Lake, Wuhan city, China. Morphologically, phage DH1 showed a typical Myoviridae structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage had a latent period of about 90 minutes and an average burst size of about 125 PFU•Cell(-1). Restriction enzyme pattern of the bacteriophage’s genome showed that the genome is a double-stranded DNA and about 34kb in size. The sequenced genomic fragments showed highly similarities to gp04 and gp16 sequence of other Myoviridae bacteriophages at protein level. Bentham Open 2015-08-31 /pmc/articles/PMC5396062/ /pubmed/28484577 http://dx.doi.org/10.2174/1874120701509010185 Text en © Kai et al.; Licensee Bentham Open. http://creativecommons.org/licenses/by-nc/3.0/ This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Kai, Cheng
Denglan, Zhang
Jingxuan, Deng
Yijun, Zhao
Isolation and Characterization of an Aeromonas punctata Bacteriophage
title Isolation and Characterization of an Aeromonas punctata Bacteriophage
title_full Isolation and Characterization of an Aeromonas punctata Bacteriophage
title_fullStr Isolation and Characterization of an Aeromonas punctata Bacteriophage
title_full_unstemmed Isolation and Characterization of an Aeromonas punctata Bacteriophage
title_short Isolation and Characterization of an Aeromonas punctata Bacteriophage
title_sort isolation and characterization of an aeromonas punctata bacteriophage
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396062/
https://www.ncbi.nlm.nih.gov/pubmed/28484577
http://dx.doi.org/10.2174/1874120701509010185
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AT yijunzhao isolationandcharacterizationofanaeromonaspunctatabacteriophage

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