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Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands

Here we describe a novel crosslinker and its application as a biotin-transfer reagent to identify cell surface receptors of soluble protein ligands on live cells. This crosslinker contains three functional groups: an aldehyde-reactive aminooxy group, a sulfhydryl, and a biotin (ASB). It is readily s...

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Detalles Bibliográficos
Autores principales: Tremblay, Tammy-Lynn, Hill, Jennifer J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396193/
https://www.ncbi.nlm.nih.gov/pubmed/28422167
http://dx.doi.org/10.1038/srep46574