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Biotin-transfer from a trifunctional crosslinker for identification of cell surface receptors of soluble protein ligands
Here we describe a novel crosslinker and its application as a biotin-transfer reagent to identify cell surface receptors of soluble protein ligands on live cells. This crosslinker contains three functional groups: an aldehyde-reactive aminooxy group, a sulfhydryl, and a biotin (ASB). It is readily s...
Autores principales: | Tremblay, Tammy-Lynn, Hill, Jennifer J. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396193/ https://www.ncbi.nlm.nih.gov/pubmed/28422167 http://dx.doi.org/10.1038/srep46574 |
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