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An enhanced recombinant amino‐terminal acetylation system and novel in vivo high‐throughput screen for molecules affecting α‐synuclein oligomerisation
Amino‐terminal acetylation is a ubiquitous protein modification affecting the majority of eukaryote proteins to regulate stability and function. We describe an optimised recombinant expression system for rapid production of amino terminal‐acetylated proteins within bacteria. We go on to describe the...
Autores principales: | Eastwood, Tara A., Baker, Karen, Brooker, Holly R., Frank, Stefanie, Mulvihill, Daniel P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396276/ https://www.ncbi.nlm.nih.gov/pubmed/28214355 http://dx.doi.org/10.1002/1873-3468.12597 |
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