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Na(+)/H(+) exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells

Following superficial injury, neighbouring gastric epithelial cells close the wound by rapid cell migration, a process called epithelial restitution. Na(+)/H(+) exchange (NHE) inhibitors interfere with restitution, but the role of the different NHE isoforms expressed in gastric pit cells has remaine...

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Autores principales: Paehler vor der Nolte, Anja, Chodisetti, Giriprakash, Yuan, Zhenglin, Busch, Florian, Riederer, Brigitte, Luo, Min, Yu, Yan, Menon, Manoj B., Schneider, Andreas, Stripecke, Renata, Nikolovska, Katerina, Yeruva, Sunil, Seidler, Ursula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396337/
https://www.ncbi.nlm.nih.gov/pubmed/28019659
http://dx.doi.org/10.1002/jcp.25758
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author Paehler vor der Nolte, Anja
Chodisetti, Giriprakash
Yuan, Zhenglin
Busch, Florian
Riederer, Brigitte
Luo, Min
Yu, Yan
Menon, Manoj B.
Schneider, Andreas
Stripecke, Renata
Nikolovska, Katerina
Yeruva, Sunil
Seidler, Ursula
author_facet Paehler vor der Nolte, Anja
Chodisetti, Giriprakash
Yuan, Zhenglin
Busch, Florian
Riederer, Brigitte
Luo, Min
Yu, Yan
Menon, Manoj B.
Schneider, Andreas
Stripecke, Renata
Nikolovska, Katerina
Yeruva, Sunil
Seidler, Ursula
author_sort Paehler vor der Nolte, Anja
collection PubMed
description Following superficial injury, neighbouring gastric epithelial cells close the wound by rapid cell migration, a process called epithelial restitution. Na(+)/H(+) exchange (NHE) inhibitors interfere with restitution, but the role of the different NHE isoforms expressed in gastric pit cells has remained elusive. The role of the basolaterally expressed NHE1 (Slc9a1) and the presumably apically expressed NHE2 (Slc9a2) in epithelial restitution was investigated in the nontransformed rat gastric surface cell line RGM1. Migration velocity was assessed by loading the cells with the fluorescent dye DiR and following closure of an experimental wound over time. Since RGM1 cells expressed very low NHE2 mRNA and have low transport activity, NHE2 was introduced by lentiviral gene transfer. In medium with pH 7.4, RGM1 cells displayed slow wound healing even in the absence of growth factors and independently of NHE activity. Growth factors accelerated wound healing in a partly NHE1‐dependent fashion. Preincubation with acidic pH 7.1 stimulated restitution in a NHE1‐dependent fashion. When pH 7.1 was maintained during the restitution period, migratory speed was reduced to ∼10% of the speed at pH 7,4, and the residual restitution was further inhibited by NHE1 inhibition. Lentiviral NHE2 expression increased the steady‐state pH(i) and reduced the restitution velocity after low pH preincubation, which was reversible by pharmacological NHE2 inhibition. The results demonstrate that in RGM1 cells, migratory velocity is increased by NHE1 activation, while NHE2 activity inhibit this process. A differential activation of NHE1 and NHE2 may therefore, play a role in the initiation and completion of the epithelial restitution process.
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spelling pubmed-53963372017-04-25 Na(+)/H(+) exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells Paehler vor der Nolte, Anja Chodisetti, Giriprakash Yuan, Zhenglin Busch, Florian Riederer, Brigitte Luo, Min Yu, Yan Menon, Manoj B. Schneider, Andreas Stripecke, Renata Nikolovska, Katerina Yeruva, Sunil Seidler, Ursula J Cell Physiol Original Research Articles Following superficial injury, neighbouring gastric epithelial cells close the wound by rapid cell migration, a process called epithelial restitution. Na(+)/H(+) exchange (NHE) inhibitors interfere with restitution, but the role of the different NHE isoforms expressed in gastric pit cells has remained elusive. The role of the basolaterally expressed NHE1 (Slc9a1) and the presumably apically expressed NHE2 (Slc9a2) in epithelial restitution was investigated in the nontransformed rat gastric surface cell line RGM1. Migration velocity was assessed by loading the cells with the fluorescent dye DiR and following closure of an experimental wound over time. Since RGM1 cells expressed very low NHE2 mRNA and have low transport activity, NHE2 was introduced by lentiviral gene transfer. In medium with pH 7.4, RGM1 cells displayed slow wound healing even in the absence of growth factors and independently of NHE activity. Growth factors accelerated wound healing in a partly NHE1‐dependent fashion. Preincubation with acidic pH 7.1 stimulated restitution in a NHE1‐dependent fashion. When pH 7.1 was maintained during the restitution period, migratory speed was reduced to ∼10% of the speed at pH 7,4, and the residual restitution was further inhibited by NHE1 inhibition. Lentiviral NHE2 expression increased the steady‐state pH(i) and reduced the restitution velocity after low pH preincubation, which was reversible by pharmacological NHE2 inhibition. The results demonstrate that in RGM1 cells, migratory velocity is increased by NHE1 activation, while NHE2 activity inhibit this process. A differential activation of NHE1 and NHE2 may therefore, play a role in the initiation and completion of the epithelial restitution process. John Wiley and Sons Inc. 2017-02-21 2017-07 /pmc/articles/PMC5396337/ /pubmed/28019659 http://dx.doi.org/10.1002/jcp.25758 Text en © 2016 The Authors. Journal of Cellular Physiology Published by Wiley Periodicals Inc. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research Articles
Paehler vor der Nolte, Anja
Chodisetti, Giriprakash
Yuan, Zhenglin
Busch, Florian
Riederer, Brigitte
Luo, Min
Yu, Yan
Menon, Manoj B.
Schneider, Andreas
Stripecke, Renata
Nikolovska, Katerina
Yeruva, Sunil
Seidler, Ursula
Na(+)/H(+) exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells
title Na(+)/H(+) exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells
title_full Na(+)/H(+) exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells
title_fullStr Na(+)/H(+) exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells
title_full_unstemmed Na(+)/H(+) exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells
title_short Na(+)/H(+) exchanger NHE1 and NHE2 have opposite effects on migration velocity in rat gastric surface cells
title_sort na(+)/h(+) exchanger nhe1 and nhe2 have opposite effects on migration velocity in rat gastric surface cells
topic Original Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396337/
https://www.ncbi.nlm.nih.gov/pubmed/28019659
http://dx.doi.org/10.1002/jcp.25758
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