Genetic diversity analysis of Morchella sp.by RAPD

This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an effici...

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Detalles Bibliográficos
Autores principales: Irfan, Muhammad, Yang, Shuang, Yuxin, Luo, Sun, Jia-Xing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shiraz University 2017
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396812/
https://www.ncbi.nlm.nih.gov/pubmed/28447046
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author Irfan, Muhammad
Yang, Shuang
Yuxin, Luo
Sun, Jia-Xing
author_facet Irfan, Muhammad
Yang, Shuang
Yuxin, Luo
Sun, Jia-Xing
author_sort Irfan, Muhammad
collection PubMed
description This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymorphism in three species of morchella. Out of all eight primers tested in current study, one of them (B8) resulted 100% polymorphism among the three studied species. Based on the RAPD profile a similarity matrix was generated to construct a dendrogram for phylogenetic analysis revealing the relationship among the three species of morchella.
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spelling pubmed-53968122017-04-26 Genetic diversity analysis of Morchella sp.by RAPD Irfan, Muhammad Yang, Shuang Yuxin, Luo Sun, Jia-Xing Mol Biol Res Commun Short Communication This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymorphism in three species of morchella. Out of all eight primers tested in current study, one of them (B8) resulted 100% polymorphism among the three studied species. Based on the RAPD profile a similarity matrix was generated to construct a dendrogram for phylogenetic analysis revealing the relationship among the three species of morchella. Shiraz University 2017-03 /pmc/articles/PMC5396812/ /pubmed/28447046 Text en This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Irfan, Muhammad
Yang, Shuang
Yuxin, Luo
Sun, Jia-Xing
Genetic diversity analysis of Morchella sp.by RAPD
title Genetic diversity analysis of Morchella sp.by RAPD
title_full Genetic diversity analysis of Morchella sp.by RAPD
title_fullStr Genetic diversity analysis of Morchella sp.by RAPD
title_full_unstemmed Genetic diversity analysis of Morchella sp.by RAPD
title_short Genetic diversity analysis of Morchella sp.by RAPD
title_sort genetic diversity analysis of morchella sp.by rapd
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5396812/
https://www.ncbi.nlm.nih.gov/pubmed/28447046
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AT sunjiaxing geneticdiversityanalysisofmorchellaspbyrapd

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