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Blending DNA binding dyes to improve detection in real-time PCR

The success of real-time PCR (qPCR) analysis is partly limited by the presence of inhibitory compounds in the nucleic acid samples. For example, humic acid (HA) from soil and aqueous sediment interferes with amplification and also quenches the fluorescence of double-stranded (ds) DNA binding dyes, t...

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Detalles Bibliográficos
Autores principales:	Jansson, Linda, Koliana, Marianne, Sidstedt, Maja, Hedman, Johannes
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397098/ https://www.ncbi.nlm.nih.gov/pubmed/28459006 http://dx.doi.org/10.1016/j.btre.2017.02.002

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