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INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes
The conserved INO80 chromatin remodeling complex is involved in regulation of DNA damage repair, replication and transcription. It is commonly recruited to the transcription start region and contributes to the establishment of promoter-proximal nucleosomes. We find a substantial influence of INO80 o...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397147/ https://www.ncbi.nlm.nih.gov/pubmed/28025392 http://dx.doi.org/10.1093/nar/gkw1292 |
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author | Klopf, Eva Schmidt, Heiko A. Clauder-Münster, Sandra Steinmetz, Lars M. Schüller, Christoph |
author_facet | Klopf, Eva Schmidt, Heiko A. Clauder-Münster, Sandra Steinmetz, Lars M. Schüller, Christoph |
author_sort | Klopf, Eva |
collection | PubMed |
description | The conserved INO80 chromatin remodeling complex is involved in regulation of DNA damage repair, replication and transcription. It is commonly recruited to the transcription start region and contributes to the establishment of promoter-proximal nucleosomes. We find a substantial influence of INO80 on nucleosome dynamics and gene expression during stress induced transcription. Transcription induced by osmotic stress leads to genome-wide remodeling of promoter proximal nucleosomes. INO80 function is required for timely return of evicted nucleosomes to the 5΄ end of induced genes. Reduced INO80 function in Arp8-deficient cells leads to correlated prolonged transcription and nucleosome eviction. INO80 and the related complex SWR1 regulate incorporation of the H2A.Z isoform at promoter proximal nucleosomes. However, H2A.Z seems not to influence osmotic stress induced gene regulation. Furthermore, we show that high rates of transcription promote INO80 recruitment to promoter regions, suggesting a connection between active transcription and promoter proximal nucleosome remodeling. In addition, we find that absence of INO80 enhances bidirectional promoter activity at highly induced genes and expression of a number of stress induced transcripts. We suggest that INO80 has a direct repressive role via promoter proximal nucleosome remodeling to limit high levels of transcription in yeast. |
format | Online Article Text |
id | pubmed-5397147 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-53971472017-04-24 INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes Klopf, Eva Schmidt, Heiko A. Clauder-Münster, Sandra Steinmetz, Lars M. Schüller, Christoph Nucleic Acids Res Gene regulation, Chromatin and Epigenetics The conserved INO80 chromatin remodeling complex is involved in regulation of DNA damage repair, replication and transcription. It is commonly recruited to the transcription start region and contributes to the establishment of promoter-proximal nucleosomes. We find a substantial influence of INO80 on nucleosome dynamics and gene expression during stress induced transcription. Transcription induced by osmotic stress leads to genome-wide remodeling of promoter proximal nucleosomes. INO80 function is required for timely return of evicted nucleosomes to the 5΄ end of induced genes. Reduced INO80 function in Arp8-deficient cells leads to correlated prolonged transcription and nucleosome eviction. INO80 and the related complex SWR1 regulate incorporation of the H2A.Z isoform at promoter proximal nucleosomes. However, H2A.Z seems not to influence osmotic stress induced gene regulation. Furthermore, we show that high rates of transcription promote INO80 recruitment to promoter regions, suggesting a connection between active transcription and promoter proximal nucleosome remodeling. In addition, we find that absence of INO80 enhances bidirectional promoter activity at highly induced genes and expression of a number of stress induced transcripts. We suggest that INO80 has a direct repressive role via promoter proximal nucleosome remodeling to limit high levels of transcription in yeast. Oxford University Press 2017-04-20 2016-12-26 /pmc/articles/PMC5397147/ /pubmed/28025392 http://dx.doi.org/10.1093/nar/gkw1292 Text en © The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Gene regulation, Chromatin and Epigenetics Klopf, Eva Schmidt, Heiko A. Clauder-Münster, Sandra Steinmetz, Lars M. Schüller, Christoph INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes |
title | INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes |
title_full | INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes |
title_fullStr | INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes |
title_full_unstemmed | INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes |
title_short | INO80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes |
title_sort | ino80 represses osmostress induced gene expression by resetting promoter proximal nucleosomes |
topic | Gene regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397147/ https://www.ncbi.nlm.nih.gov/pubmed/28025392 http://dx.doi.org/10.1093/nar/gkw1292 |
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