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Simultaneous detection of mRNA transcription and decay intermediates by dual colour single mRNA FISH on subcellular resolution
The detection of mRNAs undergoing transcription or decay is challenging, because both processes are fast. However, the relative proportion of an mRNA in synthesis or decay increases with mRNA size and decreases with mRNA half-life. Based on this rationale, I have exploited a 22 200 nucleotide-long,...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Oxford University Press
2017
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397161/ https://www.ncbi.nlm.nih.gov/pubmed/27940558 http://dx.doi.org/10.1093/nar/gkw1245 |