Cargando…

pBACode: a random-barcode-based high-throughput approach for BAC paired-end sequencing and physical clone mapping

Applications that use Bacterial Artificial Chromosome (BAC) libraries often require paired-end sequences and knowledge of the physical location of each clone in plates. To facilitate obtaining this information in high-throughput, we generated pBACode vectors: a pool of BAC cloning vectors, each with...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wei, Xiaolin, Xu, Zhichao, Wang, Guixing, Hou, Jilun, Ma, Xiaopeng, Liu, Haijin, Liu, Jiadong, Chen, Bo, Luo, Meizhong, Xie, Bingyan, Li, Ruiqiang, Ruan, Jue, Liu, Xiao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2017
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397170/ https://www.ncbi.nlm.nih.gov/pubmed/27980066 http://dx.doi.org/10.1093/nar/gkw1261

Ejemplares similares

Table of Contents & Bacode
Publicado: (2020)

An improved method to identify BAC clones using pooled overgos
por: Madishetty, Kavitha, et al.
Publicado: (2007)

Using high-throughput barcode sequencing to efficiently map connectomes
por: Peikon, Ian D., et al.
Publicado: (2017)

Genomic resources of broomcorn millet: demonstration and application of a high-throughput BAC mapping pipeline
por: Xu, Wei, et al.
Publicado: (2021)

Why barcode? High-throughput multiplex sequencing of mitochondrial genomes for molecular systematics
por: Timmermans, M. J. T. N., et al.
Publicado: (2010)

Suppression of artifacts and barcode bias in high-throughput transcriptome analyses utilizing template switching
por: Tang, Dave T. P., et al.
Publicado: (2013)

Utilization of Super BAC Pools and Fluidigm Access Array Platform for High-Throughput BAC Clone Identification: Proof of Concept
por: Maughan, Peter J., et al.
Publicado: (2012)

A novel high-throughput (HTP) cloning strategy for site-directed designed chimeragenesis and mutation using the Gateway cloning system
por: Suzuki, Yasuhiro, et al.
Publicado: (2005)

Solid-phase cloning for high-throughput assembly of single and multiple DNA parts
por: Lundqvist, Magnus, et al.
Publicado: (2015)

Generation of an inducible and optimized piggyBac transposon system(†)
por: Cadiñanos, Juan, et al.
Publicado: (2007)

Mobilization of giant piggyBac transposons in the mouse genome
por: Li, Meng Amy, et al.
Publicado: (2011)

Transposon-mediated BAC transgenesis in human ES cells
por: Rostovskaya, Maria, et al.
Publicado: (2012)

A pair of new BAC and BIBAC vectors that facilitate BAC/BIBAC library construction and intact large genomic DNA insert exchange
por: Shi, Xue, et al.
Publicado: (2011)

A versatile ligation-independent cloning method suitable for high-throughput expression screening applications
por: Berrow, Nick S., et al.
Publicado: (2007)

A dual-fluorescence reporter system for high-throughput clone characterization and selection by cell sorting
por: Choe, Juno, et al.
Publicado: (2005)

Screening and chromosome localization of two cotton BAC clones
por: Cui, Xinglei, et al.
Publicado: (2016)

Precise BAC targeting of genetically polymorphic mouse ES cells
por: Barakat, Tahsin Stefan, et al.
Publicado: (2011)

PiggyBac transgenic strategies in the developing chicken spinal cord
por: Lu, Yanyan, et al.
Publicado: (2009)

An optimized, broadly applicable piggyBac transposon induction system
por: Qi, Zongtai, et al.
Publicado: (2017)

Simple and highly efficient BAC recombineering using galK selection
por: Warming, Søren, et al.
Publicado: (2005)

A new implementation of high-throughput five-dimensional clone pooling strategy for BAC library screening
por: You, Frank M, et al.
Publicado: (2010)

High-resolution nucleosome mapping of targeted regions using BAC-based enrichment
por: Yigit, Erbay, et al.
Publicado: (2013)

A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe
por: Li, Jun, et al.
Publicado: (2011)

DNA transposition by protein transduction of the piggyBac transposase from lentiviral Gag precursors
por: Cai, Yujia, et al.
Publicado: (2014)

Advantages of q-PCR as a method of screening for gene targeting in mammalian cells using conventional and whole BAC-based constructs
por: Gómez-Rodríguez, Julio, et al.
Publicado: (2008)

Production and verification of a 2(nd) generation clonal group of Japanese flounder, Paralichthys olivaceus
por: Hou, Jilun, et al.
Publicado: (2016)

Construction of a BAC library and mapping BAC clones to the linkage map of Barramundi, Lates calcarifer
por: Wang, Chun Ming, et al.
Publicado: (2008)

Counter-selection recombineering of the baculovirus genome: a strategy for seamless modification of repeat-containing BACs
por: Westenberg, Marcel, et al.
Publicado: (2010)

Direct cloning of a herpesvirus genome for rapid generation of infectious BAC clones
por: Yuan, Hengxing, et al.
Publicado: (2022)

A BAC based physical map and genome survey of the rice false smut fungus Villosiclava virens
por: Wang, Xiaoming, et al.
Publicado: (2013)

Barcoded oligonucleotides ligated on RNA amplified for multiplexed and parallel in situ analyses
por: Liu, Songlei, et al.
Publicado: (2021)

A high‐throughput BAC end analysis protocol (BAC‐anchor) for profiling genome assembly and physical mapping
por: Yang, Xiaohui, et al.
Publicado: (2019)

Multiplexing clonality: combining RGB marking and genetic barcoding
por: Cornils, Kerstin, et al.
Publicado: (2014)

In vivo generation of DNA sequence diversity for cellular barcoding
por: Peikon, Ian D., et al.
Publicado: (2014)

Targeting individual cells by barcode in pooled sequence libraries
por: Ranu, Navpreet, et al.
Publicado: (2019)

HaloTag-based conjugation of proteins to barcoding-oligonucleotides
por: Yazaki, Junshi, et al.
Publicado: (2020)

Slingshot: a PiggyBac based transposon system for tamoxifen-inducible ‘self-inactivating’ insertional mutagenesis
por: Kong, Jun, et al.
Publicado: (2010)

Efficient conditional knockout targeting vector construction using co-selection BAC recombineering (CoSBR)
por: Newman, Robert J., et al.
Publicado: (2015)

Correction: Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder
por: Zhang, Xiaoyan, et al.
Publicado: (2016)

Gonadal Transcriptome Analysis in Sterile Double Haploid Japanese Flounder
por: Zhang, Xiaoyan, et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_9cqukgtitv6t65bh351l0kudn4