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Measurements of translation initiation from all 64 codons in E. coli

Our understanding of translation underpins our capacity to engineer living systems. The canonical start codon (AUG) and a few near-cognates (GUG, UUG) are considered as the ‘start codons’ for translation initiation in Escherichia coli. Translation is typically not thought to initiate from the 61 rem...

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Autores principales: Hecht, Ariel, Glasgow, Jeff, Jaschke, Paul R., Bawazer, Lukmaan A., Munson, Matthew S., Cochran, Jennifer R., Endy, Drew, Salit, Marc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397182/
https://www.ncbi.nlm.nih.gov/pubmed/28334756
http://dx.doi.org/10.1093/nar/gkx070
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author Hecht, Ariel
Glasgow, Jeff
Jaschke, Paul R.
Bawazer, Lukmaan A.
Munson, Matthew S.
Cochran, Jennifer R.
Endy, Drew
Salit, Marc
author_facet Hecht, Ariel
Glasgow, Jeff
Jaschke, Paul R.
Bawazer, Lukmaan A.
Munson, Matthew S.
Cochran, Jennifer R.
Endy, Drew
Salit, Marc
author_sort Hecht, Ariel
collection PubMed
description Our understanding of translation underpins our capacity to engineer living systems. The canonical start codon (AUG) and a few near-cognates (GUG, UUG) are considered as the ‘start codons’ for translation initiation in Escherichia coli. Translation is typically not thought to initiate from the 61 remaining codons. Here, we quantified translation initiation of green fluorescent protein and nanoluciferase in E. coli from all 64 triplet codons and across a range of DNA copy number. We detected initiation of protein synthesis above measurement background for 47 codons. Translation from non-canonical start codons ranged from 0.007 to 3% relative to translation from AUG. Translation from 17 non-AUG codons exceeded the highest reported rates of non-cognate codon recognition. Translation initiation from non-canonical start codons may contribute to the synthesis of peptides in both natural and synthetic biological systems.
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spelling pubmed-53971822017-04-24 Measurements of translation initiation from all 64 codons in E. coli Hecht, Ariel Glasgow, Jeff Jaschke, Paul R. Bawazer, Lukmaan A. Munson, Matthew S. Cochran, Jennifer R. Endy, Drew Salit, Marc Nucleic Acids Res NAR Breakthrough Article Our understanding of translation underpins our capacity to engineer living systems. The canonical start codon (AUG) and a few near-cognates (GUG, UUG) are considered as the ‘start codons’ for translation initiation in Escherichia coli. Translation is typically not thought to initiate from the 61 remaining codons. Here, we quantified translation initiation of green fluorescent protein and nanoluciferase in E. coli from all 64 triplet codons and across a range of DNA copy number. We detected initiation of protein synthesis above measurement background for 47 codons. Translation from non-canonical start codons ranged from 0.007 to 3% relative to translation from AUG. Translation from 17 non-AUG codons exceeded the highest reported rates of non-cognate codon recognition. Translation initiation from non-canonical start codons may contribute to the synthesis of peptides in both natural and synthetic biological systems. Oxford University Press 2017-04-20 2017-02-21 /pmc/articles/PMC5397182/ /pubmed/28334756 http://dx.doi.org/10.1093/nar/gkx070 Text en Published by Oxford University Press on behalf of Nucleic Acids Research 2017. This work is written by (a) US Government employee(s) and is in the public domain in the US.
spellingShingle NAR Breakthrough Article
Hecht, Ariel
Glasgow, Jeff
Jaschke, Paul R.
Bawazer, Lukmaan A.
Munson, Matthew S.
Cochran, Jennifer R.
Endy, Drew
Salit, Marc
Measurements of translation initiation from all 64 codons in E. coli
title Measurements of translation initiation from all 64 codons in E. coli
title_full Measurements of translation initiation from all 64 codons in E. coli
title_fullStr Measurements of translation initiation from all 64 codons in E. coli
title_full_unstemmed Measurements of translation initiation from all 64 codons in E. coli
title_short Measurements of translation initiation from all 64 codons in E. coli
title_sort measurements of translation initiation from all 64 codons in e. coli
topic NAR Breakthrough Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5397182/
https://www.ncbi.nlm.nih.gov/pubmed/28334756
http://dx.doi.org/10.1093/nar/gkx070
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