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Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings

This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric po...

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Autores principales: Aanangi, Raveendra, Kotapati, Kasi Viswanath, Palaka, Bhagath Kumar, Kedam, Thyagaraju, Kanika, Nirmala Devi, Ampasala, Dinakara Rao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5398193/
https://www.ncbi.nlm.nih.gov/pubmed/28330183
http://dx.doi.org/10.1007/s13205-016-0427-5
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author Aanangi, Raveendra
Kotapati, Kasi Viswanath
Palaka, Bhagath Kumar
Kedam, Thyagaraju
Kanika, Nirmala Devi
Ampasala, Dinakara Rao
author_facet Aanangi, Raveendra
Kotapati, Kasi Viswanath
Palaka, Bhagath Kumar
Kedam, Thyagaraju
Kanika, Nirmala Devi
Ampasala, Dinakara Rao
author_sort Aanangi, Raveendra
collection PubMed
description This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric point of 5.84 and the other has lowest LOX activity with an isoelectric point of 5.52. The purified LOX has molecular mass of approximately 97 kDa and showed high activity with linoleic acid than linolenic acid and arachidonic acid. The optimal activity of LOX was observed at pH 6.5 and temperature 35 °C. Far-UV circular dichroism (CD) studies revealed that the purified mung bean LOX possess secondary structural elements with significant α-helix and β-strands. Further, the secondary structure of mung bean LOX was stable up to 60 °C at pH 6.5. Biophysical and chemical properties of the mung bean LOX are similar to the other legume LOXs and may be considered as type-1 LOX.
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spelling pubmed-53981932017-04-21 Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings Aanangi, Raveendra Kotapati, Kasi Viswanath Palaka, Bhagath Kumar Kedam, Thyagaraju Kanika, Nirmala Devi Ampasala, Dinakara Rao 3 Biotech Original Article This study reports purification and characterization of lipoxygenase protein from mung bean germinating seedlings. Lipoxygenases (LOXs) are key enzymes in seed germination. The purified mung bean LOX has resolved into two peaks by chromatofocusing, one has highest LOX activity with an isoelectric point of 5.84 and the other has lowest LOX activity with an isoelectric point of 5.52. The purified LOX has molecular mass of approximately 97 kDa and showed high activity with linoleic acid than linolenic acid and arachidonic acid. The optimal activity of LOX was observed at pH 6.5 and temperature 35 °C. Far-UV circular dichroism (CD) studies revealed that the purified mung bean LOX possess secondary structural elements with significant α-helix and β-strands. Further, the secondary structure of mung bean LOX was stable up to 60 °C at pH 6.5. Biophysical and chemical properties of the mung bean LOX are similar to the other legume LOXs and may be considered as type-1 LOX. Springer Berlin Heidelberg 2016-05-17 2016-06 /pmc/articles/PMC5398193/ /pubmed/28330183 http://dx.doi.org/10.1007/s13205-016-0427-5 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Aanangi, Raveendra
Kotapati, Kasi Viswanath
Palaka, Bhagath Kumar
Kedam, Thyagaraju
Kanika, Nirmala Devi
Ampasala, Dinakara Rao
Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
title Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
title_full Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
title_fullStr Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
title_full_unstemmed Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
title_short Purification and characterization of lipoxygenase from mung bean (Vigna radiata L.) germinating seedlings
title_sort purification and characterization of lipoxygenase from mung bean (vigna radiata l.) germinating seedlings
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5398193/
https://www.ncbi.nlm.nih.gov/pubmed/28330183
http://dx.doi.org/10.1007/s13205-016-0427-5
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