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Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b
Research on regulation and its mechanism of bone marrow mesenchymal stem cells (BMSCs) on dendritic cells (DCs), which is the initiating factor in immune response has applicable clinical value. Although BMSCs have a significant regulatory effect on the maturation of DCs, its molecular mechanism is s...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5398252/ https://www.ncbi.nlm.nih.gov/pubmed/28096318 http://dx.doi.org/10.1042/BSR20160436 |
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author | Wu, Jingguo Ji, Chengbo Cao, Feifei Lui, Hongfen Xia, Bo Wang, Lanping |
author_facet | Wu, Jingguo Ji, Chengbo Cao, Feifei Lui, Hongfen Xia, Bo Wang, Lanping |
author_sort | Wu, Jingguo |
collection | PubMed |
description | Research on regulation and its mechanism of bone marrow mesenchymal stem cells (BMSCs) on dendritic cells (DCs), which is the initiating factor in immune response has applicable clinical value. Although BMSCs have a significant regulatory effect on the maturation of DCs, its molecular mechanism is still unclear. BMSCs and DCs, were co-cultured by different concentration ratios. Flow cytometry was used to detect the expression of DC markers (CD83, CD11c). Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression of related genes in RNA level. Expression of the target proteins was detected with using Western blot assay. miRNA inhibitor and miRNA mimic were used to suppress and up-regulate the expression of the target gene. In this research, our results demonstrated that BMSCs notably inhibited maturation of DCs in the co-culture system of BMSCs and DCs and confirmed that this inhibition is due to overexpression of miR-23b. Furthermore, this research found that miR-23b overexpression inhibited the expression of p50/p65, thus blocked the activation of the NF-κB pathway. In conclusion, BMSCs affected the activation of NF-κB pathway through miR-23b overexpression resulting in inhibition of the maturation and differentiation of DCs. |
format | Online Article Text |
id | pubmed-5398252 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-53982522017-05-04 Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b Wu, Jingguo Ji, Chengbo Cao, Feifei Lui, Hongfen Xia, Bo Wang, Lanping Biosci Rep Research Articles Research on regulation and its mechanism of bone marrow mesenchymal stem cells (BMSCs) on dendritic cells (DCs), which is the initiating factor in immune response has applicable clinical value. Although BMSCs have a significant regulatory effect on the maturation of DCs, its molecular mechanism is still unclear. BMSCs and DCs, were co-cultured by different concentration ratios. Flow cytometry was used to detect the expression of DC markers (CD83, CD11c). Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression of related genes in RNA level. Expression of the target proteins was detected with using Western blot assay. miRNA inhibitor and miRNA mimic were used to suppress and up-regulate the expression of the target gene. In this research, our results demonstrated that BMSCs notably inhibited maturation of DCs in the co-culture system of BMSCs and DCs and confirmed that this inhibition is due to overexpression of miR-23b. Furthermore, this research found that miR-23b overexpression inhibited the expression of p50/p65, thus blocked the activation of the NF-κB pathway. In conclusion, BMSCs affected the activation of NF-κB pathway through miR-23b overexpression resulting in inhibition of the maturation and differentiation of DCs. Portland Press Ltd. 2017-04-20 /pmc/articles/PMC5398252/ /pubmed/28096318 http://dx.doi.org/10.1042/BSR20160436 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Wu, Jingguo Ji, Chengbo Cao, Feifei Lui, Hongfen Xia, Bo Wang, Lanping Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b |
title | Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b |
title_full | Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b |
title_fullStr | Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b |
title_full_unstemmed | Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b |
title_short | Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b |
title_sort | bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microrna-23b |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5398252/ https://www.ncbi.nlm.nih.gov/pubmed/28096318 http://dx.doi.org/10.1042/BSR20160436 |
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