Cargando…

Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b

Research on regulation and its mechanism of bone marrow mesenchymal stem cells (BMSCs) on dendritic cells (DCs), which is the initiating factor in immune response has applicable clinical value. Although BMSCs have a significant regulatory effect on the maturation of DCs, its molecular mechanism is s...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Jingguo, Ji, Chengbo, Cao, Feifei, Lui, Hongfen, Xia, Bo, Wang, Lanping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5398252/
https://www.ncbi.nlm.nih.gov/pubmed/28096318
http://dx.doi.org/10.1042/BSR20160436
_version_ 1783230427577712640
author Wu, Jingguo
Ji, Chengbo
Cao, Feifei
Lui, Hongfen
Xia, Bo
Wang, Lanping
author_facet Wu, Jingguo
Ji, Chengbo
Cao, Feifei
Lui, Hongfen
Xia, Bo
Wang, Lanping
author_sort Wu, Jingguo
collection PubMed
description Research on regulation and its mechanism of bone marrow mesenchymal stem cells (BMSCs) on dendritic cells (DCs), which is the initiating factor in immune response has applicable clinical value. Although BMSCs have a significant regulatory effect on the maturation of DCs, its molecular mechanism is still unclear. BMSCs and DCs, were co-cultured by different concentration ratios. Flow cytometry was used to detect the expression of DC markers (CD83, CD11c). Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression of related genes in RNA level. Expression of the target proteins was detected with using Western blot assay. miRNA inhibitor and miRNA mimic were used to suppress and up-regulate the expression of the target gene. In this research, our results demonstrated that BMSCs notably inhibited maturation of DCs in the co-culture system of BMSCs and DCs and confirmed that this inhibition is due to overexpression of miR-23b. Furthermore, this research found that miR-23b overexpression inhibited the expression of p50/p65, thus blocked the activation of the NF-κB pathway. In conclusion, BMSCs affected the activation of NF-κB pathway through miR-23b overexpression resulting in inhibition of the maturation and differentiation of DCs.
format Online
Article
Text
id pubmed-5398252
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Portland Press Ltd.
record_format MEDLINE/PubMed
spelling pubmed-53982522017-05-04 Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b Wu, Jingguo Ji, Chengbo Cao, Feifei Lui, Hongfen Xia, Bo Wang, Lanping Biosci Rep Research Articles Research on regulation and its mechanism of bone marrow mesenchymal stem cells (BMSCs) on dendritic cells (DCs), which is the initiating factor in immune response has applicable clinical value. Although BMSCs have a significant regulatory effect on the maturation of DCs, its molecular mechanism is still unclear. BMSCs and DCs, were co-cultured by different concentration ratios. Flow cytometry was used to detect the expression of DC markers (CD83, CD11c). Quantitative reverse transcription PCR (qRT-PCR) was used to measure the expression of related genes in RNA level. Expression of the target proteins was detected with using Western blot assay. miRNA inhibitor and miRNA mimic were used to suppress and up-regulate the expression of the target gene. In this research, our results demonstrated that BMSCs notably inhibited maturation of DCs in the co-culture system of BMSCs and DCs and confirmed that this inhibition is due to overexpression of miR-23b. Furthermore, this research found that miR-23b overexpression inhibited the expression of p50/p65, thus blocked the activation of the NF-κB pathway. In conclusion, BMSCs affected the activation of NF-κB pathway through miR-23b overexpression resulting in inhibition of the maturation and differentiation of DCs. Portland Press Ltd. 2017-04-20 /pmc/articles/PMC5398252/ /pubmed/28096318 http://dx.doi.org/10.1042/BSR20160436 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Wu, Jingguo
Ji, Chengbo
Cao, Feifei
Lui, Hongfen
Xia, Bo
Wang, Lanping
Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b
title Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b
title_full Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b
title_fullStr Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b
title_full_unstemmed Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b
title_short Bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microRNA-23b
title_sort bone marrow mesenchymal stem cells inhibit dendritic cells differentiation and maturation by microrna-23b
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5398252/
https://www.ncbi.nlm.nih.gov/pubmed/28096318
http://dx.doi.org/10.1042/BSR20160436
work_keys_str_mv AT wujingguo bonemarrowmesenchymalstemcellsinhibitdendriticcellsdifferentiationandmaturationbymicrorna23b
AT jichengbo bonemarrowmesenchymalstemcellsinhibitdendriticcellsdifferentiationandmaturationbymicrorna23b
AT caofeifei bonemarrowmesenchymalstemcellsinhibitdendriticcellsdifferentiationandmaturationbymicrorna23b
AT luihongfen bonemarrowmesenchymalstemcellsinhibitdendriticcellsdifferentiationandmaturationbymicrorna23b
AT xiabo bonemarrowmesenchymalstemcellsinhibitdendriticcellsdifferentiationandmaturationbymicrorna23b
AT wanglanping bonemarrowmesenchymalstemcellsinhibitdendriticcellsdifferentiationandmaturationbymicrorna23b