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Expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges

The deposition of toxic munitions compounds, such as hexahydro‐1, 3, 5‐trinitro‐1, 3, 5‐triazine (RDX), on soils around targets in live‐fire training ranges is an important source of groundwater contamination. Plants take up RDX but do not significantly degrade it. Reported here is the transformatio...

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Autores principales: Zhang, Long, Routsong, Ryan, Nguyen, Quyen, Rylott, Elizabeth L., Bruce, Neil C., Strand, Stuart E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399000/
https://www.ncbi.nlm.nih.gov/pubmed/27862819
http://dx.doi.org/10.1111/pbi.12661
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author Zhang, Long
Routsong, Ryan
Nguyen, Quyen
Rylott, Elizabeth L.
Bruce, Neil C.
Strand, Stuart E.
author_facet Zhang, Long
Routsong, Ryan
Nguyen, Quyen
Rylott, Elizabeth L.
Bruce, Neil C.
Strand, Stuart E.
author_sort Zhang, Long
collection PubMed
description The deposition of toxic munitions compounds, such as hexahydro‐1, 3, 5‐trinitro‐1, 3, 5‐triazine (RDX), on soils around targets in live‐fire training ranges is an important source of groundwater contamination. Plants take up RDX but do not significantly degrade it. Reported here is the transformation of two perennial grass species, switchgrass (Panicum virgatum) and creeping bentgrass (Agrostis stolonifera), with the genes for degradation of RDX. These species possess a number of agronomic traits making them well equipped for the uptake and removal of RDX from root zone leachates. Transformation vectors were constructed with xplA and xplB, which confer the ability to degrade RDX, and nfsI, which encodes a nitroreductase for the detoxification of the co‐contaminating explosive 2, 4, 6‐trinitrotoluene (TNT). The vectors were transformed into the grass species using Agrobacterium tumefaciens infection. All transformed grass lines showing high transgene expression levels removed significantly more RDX from hydroponic solutions and retained significantly less RDX in their leaf tissues than wild‐type plants. Soil columns planted with the best‐performing switchgrass line were able to prevent leaching of RDX through a 0.5‐m root zone. These plants represent a promising plant biotechnology to sustainably remove RDX from training range soil, thus preventing contamination of groundwater.
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spelling pubmed-53990002017-05-15 Expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges Zhang, Long Routsong, Ryan Nguyen, Quyen Rylott, Elizabeth L. Bruce, Neil C. Strand, Stuart E. Plant Biotechnol J Research Articles The deposition of toxic munitions compounds, such as hexahydro‐1, 3, 5‐trinitro‐1, 3, 5‐triazine (RDX), on soils around targets in live‐fire training ranges is an important source of groundwater contamination. Plants take up RDX but do not significantly degrade it. Reported here is the transformation of two perennial grass species, switchgrass (Panicum virgatum) and creeping bentgrass (Agrostis stolonifera), with the genes for degradation of RDX. These species possess a number of agronomic traits making them well equipped for the uptake and removal of RDX from root zone leachates. Transformation vectors were constructed with xplA and xplB, which confer the ability to degrade RDX, and nfsI, which encodes a nitroreductase for the detoxification of the co‐contaminating explosive 2, 4, 6‐trinitrotoluene (TNT). The vectors were transformed into the grass species using Agrobacterium tumefaciens infection. All transformed grass lines showing high transgene expression levels removed significantly more RDX from hydroponic solutions and retained significantly less RDX in their leaf tissues than wild‐type plants. Soil columns planted with the best‐performing switchgrass line were able to prevent leaching of RDX through a 0.5‐m root zone. These plants represent a promising plant biotechnology to sustainably remove RDX from training range soil, thus preventing contamination of groundwater. John Wiley and Sons Inc. 2016-12-29 2017-05 /pmc/articles/PMC5399000/ /pubmed/27862819 http://dx.doi.org/10.1111/pbi.12661 Text en © 2016 The Authors. Plant Biotechnology Journal published by Society for Experimental Biology and The Association of Applied Biologists and John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Zhang, Long
Routsong, Ryan
Nguyen, Quyen
Rylott, Elizabeth L.
Bruce, Neil C.
Strand, Stuart E.
Expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges
title Expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges
title_full Expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges
title_fullStr Expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges
title_full_unstemmed Expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges
title_short Expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges
title_sort expression in grasses of multiple transgenes for degradation of munitions compounds on live‐fire training ranges
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399000/
https://www.ncbi.nlm.nih.gov/pubmed/27862819
http://dx.doi.org/10.1111/pbi.12661
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