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Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat

BACKGROUND: Gravity is omnipresent on Earth; however, humans in space, such as astronauts at the International Space Station, experience microgravity. Long-term exposure to microgravity is considered to elicit physiological changes, such as muscle atrophy, in the human body. In addition, certain typ...

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Autores principales: Kim, Yoon Jae, Jeong, Ae Jin, Kim, Myungjoon, Lee, Chiwon, Ye, Sang-Kyu, Kim, Sungwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399336/
https://www.ncbi.nlm.nih.gov/pubmed/28427408
http://dx.doi.org/10.1186/s12938-017-0337-8
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author Kim, Yoon Jae
Jeong, Ae Jin
Kim, Myungjoon
Lee, Chiwon
Ye, Sang-Kyu
Kim, Sungwan
author_facet Kim, Yoon Jae
Jeong, Ae Jin
Kim, Myungjoon
Lee, Chiwon
Ye, Sang-Kyu
Kim, Sungwan
author_sort Kim, Yoon Jae
collection PubMed
description BACKGROUND: Gravity is omnipresent on Earth; however, humans in space, such as astronauts at the International Space Station, experience microgravity. Long-term exposure to microgravity is considered to elicit physiological changes, such as muscle atrophy, in the human body. In addition, certain types of cancer cells demonstrate inhibited proliferation under condition of time-averaged simulated microgravity (taSMG). However, the response of human Hodgkin’s lymphoma cancer cells to reduced gravity, and the associated physiological changes in these cells, have not been elucidated. METHODS: In this study, the proliferation of human Hodgkin’s lymphoma cancer cells (L-540 and HDLM-2) under taSMG condition (<10(−3) G, 1 G is defined as 9.8 m/s(2)) was studied using a 3D clinostat. Normal human dermal fibroblast (HDF) was proliferated in the same condition as a control group. For the development of 3D clinostat, two motors were used to actuate the frames. Electrical wires for power supply and communication were connected via slip ring. For symmetrical path of gravitational vector, optimal angular velocities of the motors were found using simulation results. Under the condition of taSMG implemented by the 3D clinostat, proliferation of the cells was observed for 3 days. RESULTS: The results indicated that proliferation of these cancer cells was significantly (p < 0.0005) inhibited under taSMG, whereas proliferation of normal HDF cells was not affected. CONCLUSIONS: Findings in this study could be significantly valuable in developing novel strategies for selective killing of cancer cells such as lymphoma.
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spelling pubmed-53993362017-04-24 Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat Kim, Yoon Jae Jeong, Ae Jin Kim, Myungjoon Lee, Chiwon Ye, Sang-Kyu Kim, Sungwan Biomed Eng Online Research BACKGROUND: Gravity is omnipresent on Earth; however, humans in space, such as astronauts at the International Space Station, experience microgravity. Long-term exposure to microgravity is considered to elicit physiological changes, such as muscle atrophy, in the human body. In addition, certain types of cancer cells demonstrate inhibited proliferation under condition of time-averaged simulated microgravity (taSMG). However, the response of human Hodgkin’s lymphoma cancer cells to reduced gravity, and the associated physiological changes in these cells, have not been elucidated. METHODS: In this study, the proliferation of human Hodgkin’s lymphoma cancer cells (L-540 and HDLM-2) under taSMG condition (<10(−3) G, 1 G is defined as 9.8 m/s(2)) was studied using a 3D clinostat. Normal human dermal fibroblast (HDF) was proliferated in the same condition as a control group. For the development of 3D clinostat, two motors were used to actuate the frames. Electrical wires for power supply and communication were connected via slip ring. For symmetrical path of gravitational vector, optimal angular velocities of the motors were found using simulation results. Under the condition of taSMG implemented by the 3D clinostat, proliferation of the cells was observed for 3 days. RESULTS: The results indicated that proliferation of these cancer cells was significantly (p < 0.0005) inhibited under taSMG, whereas proliferation of normal HDF cells was not affected. CONCLUSIONS: Findings in this study could be significantly valuable in developing novel strategies for selective killing of cancer cells such as lymphoma. BioMed Central 2017-04-20 /pmc/articles/PMC5399336/ /pubmed/28427408 http://dx.doi.org/10.1186/s12938-017-0337-8 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Kim, Yoon Jae
Jeong, Ae Jin
Kim, Myungjoon
Lee, Chiwon
Ye, Sang-Kyu
Kim, Sungwan
Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat
title Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat
title_full Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat
title_fullStr Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat
title_full_unstemmed Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat
title_short Time-averaged simulated microgravity (taSMG) inhibits proliferation of lymphoma cells, L-540 and HDLM-2, using a 3D clinostat
title_sort time-averaged simulated microgravity (tasmg) inhibits proliferation of lymphoma cells, l-540 and hdlm-2, using a 3d clinostat
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399336/
https://www.ncbi.nlm.nih.gov/pubmed/28427408
http://dx.doi.org/10.1186/s12938-017-0337-8
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