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Serum MicroRNA profile in patients with colon adenomas or cancer

BACKGROUND: Colon cancer, one of the most common causes of cancer-related deaths, arises from adenomatous polyps. In these years, circulating microRNAs (miRNAs) have attracted increasing attention as novel biomarkers for colon cancers. The dysregulated circulating miRNAs in patients with colon adeno...

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Autores principales: Zhang, Yajie, Li, Min, Ding, Yijiang, Fan, Zhimin, Zhang, Jinchun, Zhang, Hongying, Jiang, Bin, Zhu, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399348/
https://www.ncbi.nlm.nih.gov/pubmed/28427387
http://dx.doi.org/10.1186/s12920-017-0260-7
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author Zhang, Yajie
Li, Min
Ding, Yijiang
Fan, Zhimin
Zhang, Jinchun
Zhang, Hongying
Jiang, Bin
Zhu, Yong
author_facet Zhang, Yajie
Li, Min
Ding, Yijiang
Fan, Zhimin
Zhang, Jinchun
Zhang, Hongying
Jiang, Bin
Zhu, Yong
author_sort Zhang, Yajie
collection PubMed
description BACKGROUND: Colon cancer, one of the most common causes of cancer-related deaths, arises from adenomatous polyps. In these years, circulating microRNAs (miRNAs) have attracted increasing attention as novel biomarkers for colon cancers. The dysregulated circulating miRNAs in patients with colon adenomas has not been well-understood. METHODS: Here, we aimed to identify miRNA profile in the serum of patients with colon adenomas or colon cancer by using microarray. Then we validated eight differentially expressed miRNAs (DEMs) by qRT-PCR and predicted their targets. RESULTS: We identified 26 DEMs from Adenomas versus Normal comparison (11 up-regulations and 15 down-regulations), 72 DEMs from Cancer versus Normal comparison (19 up-regulations and 53 down-regulations) and 17 DEMs from Cancer versus Adenomas comparison (4 up-regulations and 13 down-regulations). Moreover, three DEMs identified from Cancer versus Normal comparison were included in the list of DEMs identified from Cancer versus Adenomas comparison, and may be specific diagnostic biomarkers for colon cancer. Five down-regulated miRNAs identified from Cancer versus Normal comparison were included in the list of DEMs identified from Adenomas versus Normal comparison, and may be important for the development of colon polyps and cancer. CONCLUSIONS: We discovered 8 circulating miRNAs associated with colon adenomas and colon cancer, and these miRNAs may potentially serve as noninvasive screening biomarkers for colon cancer. Our study is useful for expanding our understanding in the development of colon adenomas and colon cancer, and thus provide novel insights into colon cancer pathogenesis and prevention. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12920-017-0260-7) contains supplementary material, which is available to authorized users.
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spelling pubmed-53993482017-04-24 Serum MicroRNA profile in patients with colon adenomas or cancer Zhang, Yajie Li, Min Ding, Yijiang Fan, Zhimin Zhang, Jinchun Zhang, Hongying Jiang, Bin Zhu, Yong BMC Med Genomics Research Article BACKGROUND: Colon cancer, one of the most common causes of cancer-related deaths, arises from adenomatous polyps. In these years, circulating microRNAs (miRNAs) have attracted increasing attention as novel biomarkers for colon cancers. The dysregulated circulating miRNAs in patients with colon adenomas has not been well-understood. METHODS: Here, we aimed to identify miRNA profile in the serum of patients with colon adenomas or colon cancer by using microarray. Then we validated eight differentially expressed miRNAs (DEMs) by qRT-PCR and predicted their targets. RESULTS: We identified 26 DEMs from Adenomas versus Normal comparison (11 up-regulations and 15 down-regulations), 72 DEMs from Cancer versus Normal comparison (19 up-regulations and 53 down-regulations) and 17 DEMs from Cancer versus Adenomas comparison (4 up-regulations and 13 down-regulations). Moreover, three DEMs identified from Cancer versus Normal comparison were included in the list of DEMs identified from Cancer versus Adenomas comparison, and may be specific diagnostic biomarkers for colon cancer. Five down-regulated miRNAs identified from Cancer versus Normal comparison were included in the list of DEMs identified from Adenomas versus Normal comparison, and may be important for the development of colon polyps and cancer. CONCLUSIONS: We discovered 8 circulating miRNAs associated with colon adenomas and colon cancer, and these miRNAs may potentially serve as noninvasive screening biomarkers for colon cancer. Our study is useful for expanding our understanding in the development of colon adenomas and colon cancer, and thus provide novel insights into colon cancer pathogenesis and prevention. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12920-017-0260-7) contains supplementary material, which is available to authorized users. BioMed Central 2017-04-20 /pmc/articles/PMC5399348/ /pubmed/28427387 http://dx.doi.org/10.1186/s12920-017-0260-7 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zhang, Yajie
Li, Min
Ding, Yijiang
Fan, Zhimin
Zhang, Jinchun
Zhang, Hongying
Jiang, Bin
Zhu, Yong
Serum MicroRNA profile in patients with colon adenomas or cancer
title Serum MicroRNA profile in patients with colon adenomas or cancer
title_full Serum MicroRNA profile in patients with colon adenomas or cancer
title_fullStr Serum MicroRNA profile in patients with colon adenomas or cancer
title_full_unstemmed Serum MicroRNA profile in patients with colon adenomas or cancer
title_short Serum MicroRNA profile in patients with colon adenomas or cancer
title_sort serum microrna profile in patients with colon adenomas or cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399348/
https://www.ncbi.nlm.nih.gov/pubmed/28427387
http://dx.doi.org/10.1186/s12920-017-0260-7
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