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Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs

BACKGROUND: Canine leishmaniasis caused by Leishmania infantum is a parasitic disease of great veterinary significance. Some dogs infected by L. infantum may mount a strong cellular immune response and clear the infection, while others may respond with exaggerated antibody production against the par...

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Autores principales: da Silva, Lidiane Gomes, Costa-Júnior, César Raimundo Lima, Figueiredo-Júnior, Carlos Alberto Santiago, Leal-Balbino, Tereza Cristina, Crovella, Sergio, Otranto, Domenico, Balbino, Valdir de Queiroz, Dantas-Torres, Filipe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399410/
https://www.ncbi.nlm.nih.gov/pubmed/28427438
http://dx.doi.org/10.1186/s13071-017-2130-8
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author da Silva, Lidiane Gomes
Costa-Júnior, César Raimundo Lima
Figueiredo-Júnior, Carlos Alberto Santiago
Leal-Balbino, Tereza Cristina
Crovella, Sergio
Otranto, Domenico
Balbino, Valdir de Queiroz
Dantas-Torres, Filipe
author_facet da Silva, Lidiane Gomes
Costa-Júnior, César Raimundo Lima
Figueiredo-Júnior, Carlos Alberto Santiago
Leal-Balbino, Tereza Cristina
Crovella, Sergio
Otranto, Domenico
Balbino, Valdir de Queiroz
Dantas-Torres, Filipe
author_sort da Silva, Lidiane Gomes
collection PubMed
description BACKGROUND: Canine leishmaniasis caused by Leishmania infantum is a parasitic disease of great veterinary significance. Some dogs infected by L. infantum may mount a strong cellular immune response and clear the infection, while others may respond with exaggerated antibody production against the parasite and develop an overt disease, which may be fatal, if left untreated. The initial factors triggering the polarization of the immune response towards a predominantly T-helper 1 or T-helper 2 cytokines, as well as the markers of resistance and susceptibility to L. infantum infection and disease development in dogs, are not fully understood. Herein, we assessed the association between single nucleotide polymorphisms (SNPs) in the canine β-defensin-1 (CBD1) gene and the infection by L. infantum in two dog populations from Brazil (Sobral in Ceará State and São Raimundo Nonato in Piauí State) and one dog population from Italy. RESULTS: A total of 387 dogs were assessed for L. infantum by real time PCR and 34.6% of them were positive. In CBD1 gene sequences from these positive dogs, nine polymorphic sites were detected, but only SNPs 3, 4, 7 and 8 were associated with L. infantum, in dogs from southern Italy. No association was found with dogs from Brazil. CONCLUSION: This study sets the basis for further studies on the usefulness of CBD1 as a marker of L. infantum infection susceptibility in dogs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-017-2130-8) contains supplementary material, which is available to authorized users.
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spelling pubmed-53994102017-04-24 Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs da Silva, Lidiane Gomes Costa-Júnior, César Raimundo Lima Figueiredo-Júnior, Carlos Alberto Santiago Leal-Balbino, Tereza Cristina Crovella, Sergio Otranto, Domenico Balbino, Valdir de Queiroz Dantas-Torres, Filipe Parasit Vectors Short Report BACKGROUND: Canine leishmaniasis caused by Leishmania infantum is a parasitic disease of great veterinary significance. Some dogs infected by L. infantum may mount a strong cellular immune response and clear the infection, while others may respond with exaggerated antibody production against the parasite and develop an overt disease, which may be fatal, if left untreated. The initial factors triggering the polarization of the immune response towards a predominantly T-helper 1 or T-helper 2 cytokines, as well as the markers of resistance and susceptibility to L. infantum infection and disease development in dogs, are not fully understood. Herein, we assessed the association between single nucleotide polymorphisms (SNPs) in the canine β-defensin-1 (CBD1) gene and the infection by L. infantum in two dog populations from Brazil (Sobral in Ceará State and São Raimundo Nonato in Piauí State) and one dog population from Italy. RESULTS: A total of 387 dogs were assessed for L. infantum by real time PCR and 34.6% of them were positive. In CBD1 gene sequences from these positive dogs, nine polymorphic sites were detected, but only SNPs 3, 4, 7 and 8 were associated with L. infantum, in dogs from southern Italy. No association was found with dogs from Brazil. CONCLUSION: This study sets the basis for further studies on the usefulness of CBD1 as a marker of L. infantum infection susceptibility in dogs. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13071-017-2130-8) contains supplementary material, which is available to authorized users. BioMed Central 2017-04-20 /pmc/articles/PMC5399410/ /pubmed/28427438 http://dx.doi.org/10.1186/s13071-017-2130-8 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Short Report
da Silva, Lidiane Gomes
Costa-Júnior, César Raimundo Lima
Figueiredo-Júnior, Carlos Alberto Santiago
Leal-Balbino, Tereza Cristina
Crovella, Sergio
Otranto, Domenico
Balbino, Valdir de Queiroz
Dantas-Torres, Filipe
Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs
title Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs
title_full Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs
title_fullStr Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs
title_full_unstemmed Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs
title_short Canine β-defensin-1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs
title_sort canine β-defensin-1 (cbd1) gene as a possible marker for leishmania infantum infection in dogs
topic Short Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399410/
https://www.ncbi.nlm.nih.gov/pubmed/28427438
http://dx.doi.org/10.1186/s13071-017-2130-8
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