Cargando…

Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats

BACKGROUND: This study aimed to evaluate the anti-aging effect of a newly prepared insect-derived compound, dung beetle glycosaminoglycan (GAG), given intraperitoneally to old SD rats as part of their diet for 1 month. Insect GAG administration was found to be related to a reduction in oxidative dam...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahn, Mi Young, Kim, Ban Ji, Kim, Ha Jeong, Hwang, Jae Sam, Jung, Yi-Sook, Park, Kun-Koo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399430/
https://www.ncbi.nlm.nih.gov/pubmed/28439422
http://dx.doi.org/10.1186/s40824-017-0091-9
_version_ 1783230644878311424
author Ahn, Mi Young
Kim, Ban Ji
Kim, Ha Jeong
Hwang, Jae Sam
Jung, Yi-Sook
Park, Kun-Koo
author_facet Ahn, Mi Young
Kim, Ban Ji
Kim, Ha Jeong
Hwang, Jae Sam
Jung, Yi-Sook
Park, Kun-Koo
author_sort Ahn, Mi Young
collection PubMed
description BACKGROUND: This study aimed to evaluate the anti-aging effect of a newly prepared insect-derived compound, dung beetle glycosaminoglycan (GAG), given intraperitoneally to old SD rats as part of their diet for 1 month. Insect GAG administration was found to be related to a reduction in oxidative damage, hepato-cellular biomarker levels, protein carbonyl content, and malondialdehyde concentration. The anti-aging-related molecular genetic mechanisms of dung beetle GAG are not yet fully elucidated. RESULTS: Catharsius molossus (a type of dung beetle) GAG (CaG) possessed anti-aging activities; it reduced the serum level of creatinine kinase, had aortic vasorelaxant activities and cardioprotective actions, and maintained a normal glucose level in treated rats. Microarray analysis was performed with a rat 30 K cDNA clone set array to identify the gene-expression profiles of 14-month-old SD rats treated with dung beetle glycosaminoglycan 5 mg/kg (CaG5) over a 1-month period, which was done to investigate its anti-aging effect as compared to that of either Bombus ignitus (a type of bumblebee) queen GAG 5 mg/kg (IQG5) or chondroitin sulfate 10 mg/kg. CaG5 and IQG5 had marked anti-inflammatory effects, bringing about inhibition of free fatty acid, uric acid, sGPT, IL-1 beta, and CK values. In addition, anticoagulant and antithrombotic effects were seen: the concentration of factor 1 (fibrinogen) was increased in CaG- treated rat plasma. The CaG5-treated rat group, compared to the control, displayed upregulation of 131 genes, including lipocalin 2 (Lbp) and a serine peptidase inhibitor, Kaszal type3 (Spink3), and 64 downregulated genes, including lysyl oxidase (Lox), serine dehydratase (sds), and retinol saturase (Retsat). CONCLUSION: Our data suggest that dung beetle glycosaminoglycan may be a helpful treatment for aged rats, which indicates its potential as a therapeutic biomaterial for aging.
format Online
Article
Text
id pubmed-5399430
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-53994302017-04-24 Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats Ahn, Mi Young Kim, Ban Ji Kim, Ha Jeong Hwang, Jae Sam Jung, Yi-Sook Park, Kun-Koo Biomater Res Research Article BACKGROUND: This study aimed to evaluate the anti-aging effect of a newly prepared insect-derived compound, dung beetle glycosaminoglycan (GAG), given intraperitoneally to old SD rats as part of their diet for 1 month. Insect GAG administration was found to be related to a reduction in oxidative damage, hepato-cellular biomarker levels, protein carbonyl content, and malondialdehyde concentration. The anti-aging-related molecular genetic mechanisms of dung beetle GAG are not yet fully elucidated. RESULTS: Catharsius molossus (a type of dung beetle) GAG (CaG) possessed anti-aging activities; it reduced the serum level of creatinine kinase, had aortic vasorelaxant activities and cardioprotective actions, and maintained a normal glucose level in treated rats. Microarray analysis was performed with a rat 30 K cDNA clone set array to identify the gene-expression profiles of 14-month-old SD rats treated with dung beetle glycosaminoglycan 5 mg/kg (CaG5) over a 1-month period, which was done to investigate its anti-aging effect as compared to that of either Bombus ignitus (a type of bumblebee) queen GAG 5 mg/kg (IQG5) or chondroitin sulfate 10 mg/kg. CaG5 and IQG5 had marked anti-inflammatory effects, bringing about inhibition of free fatty acid, uric acid, sGPT, IL-1 beta, and CK values. In addition, anticoagulant and antithrombotic effects were seen: the concentration of factor 1 (fibrinogen) was increased in CaG- treated rat plasma. The CaG5-treated rat group, compared to the control, displayed upregulation of 131 genes, including lipocalin 2 (Lbp) and a serine peptidase inhibitor, Kaszal type3 (Spink3), and 64 downregulated genes, including lysyl oxidase (Lox), serine dehydratase (sds), and retinol saturase (Retsat). CONCLUSION: Our data suggest that dung beetle glycosaminoglycan may be a helpful treatment for aged rats, which indicates its potential as a therapeutic biomaterial for aging. BioMed Central 2017-04-21 /pmc/articles/PMC5399430/ /pubmed/28439422 http://dx.doi.org/10.1186/s40824-017-0091-9 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ahn, Mi Young
Kim, Ban Ji
Kim, Ha Jeong
Hwang, Jae Sam
Jung, Yi-Sook
Park, Kun-Koo
Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
title Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
title_full Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
title_fullStr Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
title_full_unstemmed Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
title_short Anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
title_sort anti-aging effect and gene expression profiling of dung beetle glycosaminoglycan in aged rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399430/
https://www.ncbi.nlm.nih.gov/pubmed/28439422
http://dx.doi.org/10.1186/s40824-017-0091-9
work_keys_str_mv AT ahnmiyoung antiagingeffectandgeneexpressionprofilingofdungbeetleglycosaminoglycaninagedrats
AT kimbanji antiagingeffectandgeneexpressionprofilingofdungbeetleglycosaminoglycaninagedrats
AT kimhajeong antiagingeffectandgeneexpressionprofilingofdungbeetleglycosaminoglycaninagedrats
AT hwangjaesam antiagingeffectandgeneexpressionprofilingofdungbeetleglycosaminoglycaninagedrats
AT jungyisook antiagingeffectandgeneexpressionprofilingofdungbeetleglycosaminoglycaninagedrats
AT parkkunkoo antiagingeffectandgeneexpressionprofilingofdungbeetleglycosaminoglycaninagedrats