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Mast Cells Promote Seasonal White Adipose Beiging in Humans
Human subcutaneous (SC) white adipose tissue (WAT) increases the expression of beige adipocyte genes in the winter. Studies in rodents suggest that a number of immune mediators are important in the beiging response. We studied the seasonal beiging response in SC WAT from lean humans. We measured the...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Diabetes Association
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399616/ https://www.ncbi.nlm.nih.gov/pubmed/28250021 http://dx.doi.org/10.2337/db16-1057 |
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author | Finlin, Brian S. Zhu, Beibei Confides, Amy L. Westgate, Philip M. Harfmann, Brianna D. Dupont-Versteegden, Esther E. Kern, Philip A. |
author_facet | Finlin, Brian S. Zhu, Beibei Confides, Amy L. Westgate, Philip M. Harfmann, Brianna D. Dupont-Versteegden, Esther E. Kern, Philip A. |
author_sort | Finlin, Brian S. |
collection | PubMed |
description | Human subcutaneous (SC) white adipose tissue (WAT) increases the expression of beige adipocyte genes in the winter. Studies in rodents suggest that a number of immune mediators are important in the beiging response. We studied the seasonal beiging response in SC WAT from lean humans. We measured the gene expression of various immune cell markers and performed multivariate analysis of the gene expression data to identify genes that predict UCP1. Interleukin (IL)-4 and, unexpectedly, the mast cell marker CPA3 predicted UCP1 gene expression. Therefore, we investigated the effects of mast cells on UCP1 induction by adipocytes. TIB64 mast cells responded to cold by releasing histamine and IL-4, and this medium stimulated UCP1 expression and lipolysis by 3T3-L1 adipocytes. Pharmacological block of mast cell degranulation potently inhibited histamine release by mast cells and inhibited adipocyte UCP1 mRNA induction by conditioned medium (CM). Consistently, the histamine receptor antagonist chlorpheniramine potently inhibited adipocyte UCP1 mRNA induction by mast cell CM. Together, these data show that mast cells sense colder temperatures, release factors that promote UCP1 expression, and are an important immune cell type in the beiging response of WAT. |
format | Online Article Text |
id | pubmed-5399616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | American Diabetes Association |
record_format | MEDLINE/PubMed |
spelling | pubmed-53996162018-05-01 Mast Cells Promote Seasonal White Adipose Beiging in Humans Finlin, Brian S. Zhu, Beibei Confides, Amy L. Westgate, Philip M. Harfmann, Brianna D. Dupont-Versteegden, Esther E. Kern, Philip A. Diabetes Obesity Studies Human subcutaneous (SC) white adipose tissue (WAT) increases the expression of beige adipocyte genes in the winter. Studies in rodents suggest that a number of immune mediators are important in the beiging response. We studied the seasonal beiging response in SC WAT from lean humans. We measured the gene expression of various immune cell markers and performed multivariate analysis of the gene expression data to identify genes that predict UCP1. Interleukin (IL)-4 and, unexpectedly, the mast cell marker CPA3 predicted UCP1 gene expression. Therefore, we investigated the effects of mast cells on UCP1 induction by adipocytes. TIB64 mast cells responded to cold by releasing histamine and IL-4, and this medium stimulated UCP1 expression and lipolysis by 3T3-L1 adipocytes. Pharmacological block of mast cell degranulation potently inhibited histamine release by mast cells and inhibited adipocyte UCP1 mRNA induction by conditioned medium (CM). Consistently, the histamine receptor antagonist chlorpheniramine potently inhibited adipocyte UCP1 mRNA induction by mast cell CM. Together, these data show that mast cells sense colder temperatures, release factors that promote UCP1 expression, and are an important immune cell type in the beiging response of WAT. American Diabetes Association 2017-05 2017-03-01 /pmc/articles/PMC5399616/ /pubmed/28250021 http://dx.doi.org/10.2337/db16-1057 Text en © 2017 by the American Diabetes Association. http://www.diabetesjournals.org/content/licenseReaders may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at http://www.diabetesjournals.org/content/license. |
spellingShingle | Obesity Studies Finlin, Brian S. Zhu, Beibei Confides, Amy L. Westgate, Philip M. Harfmann, Brianna D. Dupont-Versteegden, Esther E. Kern, Philip A. Mast Cells Promote Seasonal White Adipose Beiging in Humans |
title | Mast Cells Promote Seasonal White Adipose Beiging in Humans |
title_full | Mast Cells Promote Seasonal White Adipose Beiging in Humans |
title_fullStr | Mast Cells Promote Seasonal White Adipose Beiging in Humans |
title_full_unstemmed | Mast Cells Promote Seasonal White Adipose Beiging in Humans |
title_short | Mast Cells Promote Seasonal White Adipose Beiging in Humans |
title_sort | mast cells promote seasonal white adipose beiging in humans |
topic | Obesity Studies |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399616/ https://www.ncbi.nlm.nih.gov/pubmed/28250021 http://dx.doi.org/10.2337/db16-1057 |
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