Cargando…

Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers

Michelia yunnanensis Franch., is a traditional ornamental, aromatic, and medicinal shrub that endemic to Yunnan Province in southwest China. Although the species has a large distribution pattern and is abundant in Yunnan Province, the populations are dramatically declining because of overexploitatio...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Xue, Shen, Shikang, Wu, Fuqin, Wang, Yuehua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399939/
https://www.ncbi.nlm.nih.gov/pubmed/28484472
http://dx.doi.org/10.3389/fpls.2017.00583
_version_ 1783230734445576192
author Zhang, Xue
Shen, Shikang
Wu, Fuqin
Wang, Yuehua
author_facet Zhang, Xue
Shen, Shikang
Wu, Fuqin
Wang, Yuehua
author_sort Zhang, Xue
collection PubMed
description Michelia yunnanensis Franch., is a traditional ornamental, aromatic, and medicinal shrub that endemic to Yunnan Province in southwest China. Although the species has a large distribution pattern and is abundant in Yunnan Province, the populations are dramatically declining because of overexploitation and habitat destruction. Studies on the genetic variation and demography of endemic species are necessary to develop effective conservation and management strategies. To generate such knowledge, we used 3 pairs of universal cpDNA markers and 10 pairs of microsatellite markers to assess the genetic diversity, genetic structure, and demographic history of 7 M. yunnanensis populations. We calculated a total of 88 alleles for 10 polymorphic loci and 10 haplotypes for a combined 2,089 bp of cpDNA. M. yunnanensis populations showed high genetic diversity (Ho = 0.551 for nuclear markers and Hd = 0.471 for cpDNA markers) and low genetic differentiation (F(ST) = 0.058). Geographical structure was not found among M. yunnanensis populations. Genetic distance and geographic distance were not correlated (P > 0.05), which indicated that geographic isolation is not the primary cause of the low genetic differentiation of M. yunnanensis. Additionally, M. yunnanensis populations contracted ~20,000–30,000 years ago, and no recent expansion occurred in current populations. Results indicated that the high genetic diversity of the species and within its populations holds promise for effective genetic resource management and sustainable utilization. Thus, we suggest that the conservation and management of M. yunnanensis should address exotic overexploitation and habitat destruction.
format Online
Article
Text
id pubmed-5399939
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-53999392017-05-08 Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers Zhang, Xue Shen, Shikang Wu, Fuqin Wang, Yuehua Front Plant Sci Plant Science Michelia yunnanensis Franch., is a traditional ornamental, aromatic, and medicinal shrub that endemic to Yunnan Province in southwest China. Although the species has a large distribution pattern and is abundant in Yunnan Province, the populations are dramatically declining because of overexploitation and habitat destruction. Studies on the genetic variation and demography of endemic species are necessary to develop effective conservation and management strategies. To generate such knowledge, we used 3 pairs of universal cpDNA markers and 10 pairs of microsatellite markers to assess the genetic diversity, genetic structure, and demographic history of 7 M. yunnanensis populations. We calculated a total of 88 alleles for 10 polymorphic loci and 10 haplotypes for a combined 2,089 bp of cpDNA. M. yunnanensis populations showed high genetic diversity (Ho = 0.551 for nuclear markers and Hd = 0.471 for cpDNA markers) and low genetic differentiation (F(ST) = 0.058). Geographical structure was not found among M. yunnanensis populations. Genetic distance and geographic distance were not correlated (P > 0.05), which indicated that geographic isolation is not the primary cause of the low genetic differentiation of M. yunnanensis. Additionally, M. yunnanensis populations contracted ~20,000–30,000 years ago, and no recent expansion occurred in current populations. Results indicated that the high genetic diversity of the species and within its populations holds promise for effective genetic resource management and sustainable utilization. Thus, we suggest that the conservation and management of M. yunnanensis should address exotic overexploitation and habitat destruction. Frontiers Media S.A. 2017-04-21 /pmc/articles/PMC5399939/ /pubmed/28484472 http://dx.doi.org/10.3389/fpls.2017.00583 Text en Copyright © 2017 Zhang, Shen, Wu and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Xue
Shen, Shikang
Wu, Fuqin
Wang, Yuehua
Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers
title Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers
title_full Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers
title_fullStr Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers
title_full_unstemmed Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers
title_short Inferring Genetic Variation and Demographic History of Michelia yunnanensis Franch. (Magnoliaceae) from Chloroplast DNA Sequences and Microsatellite Markers
title_sort inferring genetic variation and demographic history of michelia yunnanensis franch. (magnoliaceae) from chloroplast dna sequences and microsatellite markers
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5399939/
https://www.ncbi.nlm.nih.gov/pubmed/28484472
http://dx.doi.org/10.3389/fpls.2017.00583
work_keys_str_mv AT zhangxue inferringgeneticvariationanddemographichistoryofmicheliayunnanensisfranchmagnoliaceaefromchloroplastdnasequencesandmicrosatellitemarkers
AT shenshikang inferringgeneticvariationanddemographichistoryofmicheliayunnanensisfranchmagnoliaceaefromchloroplastdnasequencesandmicrosatellitemarkers
AT wufuqin inferringgeneticvariationanddemographichistoryofmicheliayunnanensisfranchmagnoliaceaefromchloroplastdnasequencesandmicrosatellitemarkers
AT wangyuehua inferringgeneticvariationanddemographichistoryofmicheliayunnanensisfranchmagnoliaceaefromchloroplastdnasequencesandmicrosatellitemarkers