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Comprehensive analysis of differentially expressed profiles of lncRNAs and construction of miR-133b mediated ceRNA network in colorectal cancer
BACKGROUND: Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in tumorigenesis. However, the mechanism remains largely unknown. RESULTS: Thousands of significantly dysregulated lncRNAs and mRNAs were identified by microarray. Furthermore, a miR-133b-meditated lncRNA-mRNA...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400568/ https://www.ncbi.nlm.nih.gov/pubmed/28177879 http://dx.doi.org/10.18632/oncotarget.15045 |
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author | Wu, Hao Wu, Runliu Chen, Miao Li, Daojiang Dai, Jing Zhang, Yi Gao, Kai Yu, Jun Hu, Gui Guo, Yihang Lin, Changwei Li, Xiaorong |
author_facet | Wu, Hao Wu, Runliu Chen, Miao Li, Daojiang Dai, Jing Zhang, Yi Gao, Kai Yu, Jun Hu, Gui Guo, Yihang Lin, Changwei Li, Xiaorong |
author_sort | Wu, Hao |
collection | PubMed |
description | BACKGROUND: Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in tumorigenesis. However, the mechanism remains largely unknown. RESULTS: Thousands of significantly dysregulated lncRNAs and mRNAs were identified by microarray. Furthermore, a miR-133b-meditated lncRNA-mRNA ceRNA network was revealed, a subset of which was validated in 14 paired CRC patient tumor/non-tumor samples. Gene set enrichment analysis (GSEA) results demonstrated that lncRNAs ENST00000520055 and ENST00000535511 shared KEGG pathways with miR-133b target genes. MATERIALS AND METHODS: We used microarrays to survey the lncRNA and mRNA expression profiles of colorectal cancer and para-cancer tissues. Gene Ontology (GO) and KEGG pathway enrichment analyses were performed to explore the functions of the significantly dysregulated genes. An innovate method was employed that combined analyses of two microarray data sets to construct a miR-133b-mediated lncRNA-mRNA competing endogenous RNAs (ceRNA) network. Quantitative RT-PCR analysis was used to validate part of this network. GSEA was used to predict the potential functions of these lncRNAs. CONCLUSIONS: This study identifies and validates a new method to investigate the miR-133b-mediated lncRNA-mRNA ceRNA network and lays the foundation for future investigation into the role of lncRNAs in colorectal cancer. |
format | Online Article Text |
id | pubmed-5400568 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-54005682017-05-03 Comprehensive analysis of differentially expressed profiles of lncRNAs and construction of miR-133b mediated ceRNA network in colorectal cancer Wu, Hao Wu, Runliu Chen, Miao Li, Daojiang Dai, Jing Zhang, Yi Gao, Kai Yu, Jun Hu, Gui Guo, Yihang Lin, Changwei Li, Xiaorong Oncotarget Research Paper BACKGROUND: Growing evidence suggests that long non-coding RNAs (lncRNAs) play a key role in tumorigenesis. However, the mechanism remains largely unknown. RESULTS: Thousands of significantly dysregulated lncRNAs and mRNAs were identified by microarray. Furthermore, a miR-133b-meditated lncRNA-mRNA ceRNA network was revealed, a subset of which was validated in 14 paired CRC patient tumor/non-tumor samples. Gene set enrichment analysis (GSEA) results demonstrated that lncRNAs ENST00000520055 and ENST00000535511 shared KEGG pathways with miR-133b target genes. MATERIALS AND METHODS: We used microarrays to survey the lncRNA and mRNA expression profiles of colorectal cancer and para-cancer tissues. Gene Ontology (GO) and KEGG pathway enrichment analyses were performed to explore the functions of the significantly dysregulated genes. An innovate method was employed that combined analyses of two microarray data sets to construct a miR-133b-mediated lncRNA-mRNA competing endogenous RNAs (ceRNA) network. Quantitative RT-PCR analysis was used to validate part of this network. GSEA was used to predict the potential functions of these lncRNAs. CONCLUSIONS: This study identifies and validates a new method to investigate the miR-133b-mediated lncRNA-mRNA ceRNA network and lays the foundation for future investigation into the role of lncRNAs in colorectal cancer. Impact Journals LLC 2017-02-03 /pmc/articles/PMC5400568/ /pubmed/28177879 http://dx.doi.org/10.18632/oncotarget.15045 Text en Copyright: © 2017 Wu et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Wu, Hao Wu, Runliu Chen, Miao Li, Daojiang Dai, Jing Zhang, Yi Gao, Kai Yu, Jun Hu, Gui Guo, Yihang Lin, Changwei Li, Xiaorong Comprehensive analysis of differentially expressed profiles of lncRNAs and construction of miR-133b mediated ceRNA network in colorectal cancer |
title | Comprehensive analysis of differentially expressed profiles of lncRNAs and construction of miR-133b mediated ceRNA network in colorectal cancer |
title_full | Comprehensive analysis of differentially expressed profiles of lncRNAs and construction of miR-133b mediated ceRNA network in colorectal cancer |
title_fullStr | Comprehensive analysis of differentially expressed profiles of lncRNAs and construction of miR-133b mediated ceRNA network in colorectal cancer |
title_full_unstemmed | Comprehensive analysis of differentially expressed profiles of lncRNAs and construction of miR-133b mediated ceRNA network in colorectal cancer |
title_short | Comprehensive analysis of differentially expressed profiles of lncRNAs and construction of miR-133b mediated ceRNA network in colorectal cancer |
title_sort | comprehensive analysis of differentially expressed profiles of lncrnas and construction of mir-133b mediated cerna network in colorectal cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5400568/ https://www.ncbi.nlm.nih.gov/pubmed/28177879 http://dx.doi.org/10.18632/oncotarget.15045 |
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