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Viability of Airborne Tumor Cells during Excision by Ultrasonic Device
Background. Laparoscopic surgery has become more widely used, but peritoneal dissemination and port-site metastasis have been reported to occur in these surgeries. One reason for these problems is the ultrasonically activated scalpel (UAS) used for laparoscopic surgery. This study aimed to investiga...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5401725/ https://www.ncbi.nlm.nih.gov/pubmed/28492061 http://dx.doi.org/10.1155/2017/4907576 |
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author | Hashimoto, Masakazu Kobayashi, Tsuyoshi Tashiro, Hirotaka Kuroda, Shintaro Mikuriya, Yoshihiro Abe, Tomoyuki Tanaka, Yuka Ohdan, Hideki |
author_facet | Hashimoto, Masakazu Kobayashi, Tsuyoshi Tashiro, Hirotaka Kuroda, Shintaro Mikuriya, Yoshihiro Abe, Tomoyuki Tanaka, Yuka Ohdan, Hideki |
author_sort | Hashimoto, Masakazu |
collection | PubMed |
description | Background. Laparoscopic surgery has become more widely used, but peritoneal dissemination and port-site metastasis have been reported to occur in these surgeries. One reason for these problems is the ultrasonically activated scalpel (UAS) used for laparoscopic surgery. This study aimed to investigate the viability of airborne cells released during cancer dissection using a UAS. Methods. Flank tumors measuring about 2 cm were induced in male NOD-Cg-Rag1(tm1Mom)IL2rg(tm1wjl/SzJ) mice by subcutaneous injection of 1 × 10(6) HepG2 cells. Dissection was performed with UAS (in high or low power modes) and PowerStar bipolar scissors. The mist of released tissue was collected in cell culture medium. The viability of the cellular material was assessed with trypan blue exclusion cell counting, counting after immunofluorescence staining, and flow cytometric analysis. Results. Large quantities of cellular debris were trapped in the tissue dispersed by both devices. In all experiments, there were significantly more viable cells produced by the UAS in high power mode. By using suction at the excision site, the number of viable cancer cells was reduced. Conclusions. This study demonstrates that viable cancer cells can be released into the nearby environment during tumor ablation with a UAS. |
format | Online Article Text |
id | pubmed-5401725 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-54017252017-05-10 Viability of Airborne Tumor Cells during Excision by Ultrasonic Device Hashimoto, Masakazu Kobayashi, Tsuyoshi Tashiro, Hirotaka Kuroda, Shintaro Mikuriya, Yoshihiro Abe, Tomoyuki Tanaka, Yuka Ohdan, Hideki Surg Res Pract Research Article Background. Laparoscopic surgery has become more widely used, but peritoneal dissemination and port-site metastasis have been reported to occur in these surgeries. One reason for these problems is the ultrasonically activated scalpel (UAS) used for laparoscopic surgery. This study aimed to investigate the viability of airborne cells released during cancer dissection using a UAS. Methods. Flank tumors measuring about 2 cm were induced in male NOD-Cg-Rag1(tm1Mom)IL2rg(tm1wjl/SzJ) mice by subcutaneous injection of 1 × 10(6) HepG2 cells. Dissection was performed with UAS (in high or low power modes) and PowerStar bipolar scissors. The mist of released tissue was collected in cell culture medium. The viability of the cellular material was assessed with trypan blue exclusion cell counting, counting after immunofluorescence staining, and flow cytometric analysis. Results. Large quantities of cellular debris were trapped in the tissue dispersed by both devices. In all experiments, there were significantly more viable cells produced by the UAS in high power mode. By using suction at the excision site, the number of viable cancer cells was reduced. Conclusions. This study demonstrates that viable cancer cells can be released into the nearby environment during tumor ablation with a UAS. Hindawi 2017 2017-04-09 /pmc/articles/PMC5401725/ /pubmed/28492061 http://dx.doi.org/10.1155/2017/4907576 Text en Copyright © 2017 Masakazu Hashimoto et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Hashimoto, Masakazu Kobayashi, Tsuyoshi Tashiro, Hirotaka Kuroda, Shintaro Mikuriya, Yoshihiro Abe, Tomoyuki Tanaka, Yuka Ohdan, Hideki Viability of Airborne Tumor Cells during Excision by Ultrasonic Device |
title | Viability of Airborne Tumor Cells during Excision by Ultrasonic Device |
title_full | Viability of Airborne Tumor Cells during Excision by Ultrasonic Device |
title_fullStr | Viability of Airborne Tumor Cells during Excision by Ultrasonic Device |
title_full_unstemmed | Viability of Airborne Tumor Cells during Excision by Ultrasonic Device |
title_short | Viability of Airborne Tumor Cells during Excision by Ultrasonic Device |
title_sort | viability of airborne tumor cells during excision by ultrasonic device |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5401725/ https://www.ncbi.nlm.nih.gov/pubmed/28492061 http://dx.doi.org/10.1155/2017/4907576 |
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