Cargando…

Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation

Lignin, one of the most abundant renewable feedstock, is used to develop a biocompatible hydrogel as anti-infective ointment. A hydrophilic polyoxazoline chain is grafted through ring opening polymerization, possess homogeneous spherical nanoparticles of 10–15 nm. The copolymer was covalently modifi...

Descripción completa

Detalles Bibliográficos
Autores principales: Mahata, Denial, Jana, Malabendu, Jana, Arundhuti, Mukherjee, Abhishek, Mondal, Nibendu, Saha, Tilak, Sen, Subhajit, Nando, Golok B., Mukhopadhyay, Chinmay K., Chakraborty, Ranadhir, Mandal, Santi M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5401907/
https://www.ncbi.nlm.nih.gov/pubmed/28401944
http://dx.doi.org/10.1038/srep46412
_version_ 1783231129781796864
author Mahata, Denial
Jana, Malabendu
Jana, Arundhuti
Mukherjee, Abhishek
Mondal, Nibendu
Saha, Tilak
Sen, Subhajit
Nando, Golok B.
Mukhopadhyay, Chinmay K.
Chakraborty, Ranadhir
Mandal, Santi M.
author_facet Mahata, Denial
Jana, Malabendu
Jana, Arundhuti
Mukherjee, Abhishek
Mondal, Nibendu
Saha, Tilak
Sen, Subhajit
Nando, Golok B.
Mukhopadhyay, Chinmay K.
Chakraborty, Ranadhir
Mandal, Santi M.
author_sort Mahata, Denial
collection PubMed
description Lignin, one of the most abundant renewable feedstock, is used to develop a biocompatible hydrogel as anti-infective ointment. A hydrophilic polyoxazoline chain is grafted through ring opening polymerization, possess homogeneous spherical nanoparticles of 10–15 nm. The copolymer was covalently modified with triazole moiety to fortify the antimicrobial and antibiofilm activities. The hydrogel was capable of down regulating the expression level of IL-1β in LPS induced macrophage cells, and to cause significant reduction of iNOS production. It supported cellular anti-inflammatory activity which was confirmed with luciferase assay, western blot, and NF-κB analysis. This novel lignin-based hydrogel tested in-vivo has shown the abilities to prevent infection of burn wound, aid healing, and an anti-inflammatory dressing material. The hydrogel reported here provides a new material platform to introduce a cost-effective and efficient ointment option after undertaking further work to look at its use in the area of clinical practice.
format Online
Article
Text
id pubmed-5401907
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-54019072017-04-26 Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation Mahata, Denial Jana, Malabendu Jana, Arundhuti Mukherjee, Abhishek Mondal, Nibendu Saha, Tilak Sen, Subhajit Nando, Golok B. Mukhopadhyay, Chinmay K. Chakraborty, Ranadhir Mandal, Santi M. Sci Rep Article Lignin, one of the most abundant renewable feedstock, is used to develop a biocompatible hydrogel as anti-infective ointment. A hydrophilic polyoxazoline chain is grafted through ring opening polymerization, possess homogeneous spherical nanoparticles of 10–15 nm. The copolymer was covalently modified with triazole moiety to fortify the antimicrobial and antibiofilm activities. The hydrogel was capable of down regulating the expression level of IL-1β in LPS induced macrophage cells, and to cause significant reduction of iNOS production. It supported cellular anti-inflammatory activity which was confirmed with luciferase assay, western blot, and NF-κB analysis. This novel lignin-based hydrogel tested in-vivo has shown the abilities to prevent infection of burn wound, aid healing, and an anti-inflammatory dressing material. The hydrogel reported here provides a new material platform to introduce a cost-effective and efficient ointment option after undertaking further work to look at its use in the area of clinical practice. Nature Publishing Group 2017-04-12 /pmc/articles/PMC5401907/ /pubmed/28401944 http://dx.doi.org/10.1038/srep46412 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Mahata, Denial
Jana, Malabendu
Jana, Arundhuti
Mukherjee, Abhishek
Mondal, Nibendu
Saha, Tilak
Sen, Subhajit
Nando, Golok B.
Mukhopadhyay, Chinmay K.
Chakraborty, Ranadhir
Mandal, Santi M.
Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation
title Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation
title_full Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation
title_fullStr Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation
title_full_unstemmed Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation
title_short Lignin-graft-Polyoxazoline Conjugated Triazole a Novel Anti-Infective Ointment to Control Persistent Inflammation
title_sort lignin-graft-polyoxazoline conjugated triazole a novel anti-infective ointment to control persistent inflammation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5401907/
https://www.ncbi.nlm.nih.gov/pubmed/28401944
http://dx.doi.org/10.1038/srep46412
work_keys_str_mv AT mahatadenial ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT janamalabendu ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT janaarundhuti ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT mukherjeeabhishek ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT mondalnibendu ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT sahatilak ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT sensubhajit ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT nandogolokb ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT mukhopadhyaychinmayk ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT chakrabortyranadhir ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation
AT mandalsantim ligningraftpolyoxazolineconjugatedtriazoleanovelantiinfectiveointmenttocontrolpersistentinflammation