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Molecular characterization of an Akabane virus isolate from West Java, Indonesia

We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on...

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Detalles Bibliográficos
Autores principales: PURNOMO EDI, Suryo, IBRAHIM, Afif, SUKOCO, Rinto, BUNALI, Lukman, TAGUCHI, Masaji, KATO, Tomoko, YANASE, Tohru, SHIRAFUJI, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402201/
https://www.ncbi.nlm.nih.gov/pubmed/28302930
http://dx.doi.org/10.1292/jvms.17-0009
Descripción
Sumario:We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia.