Molecular characterization of an Akabane virus isolate from West Java, Indonesia

We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on...

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Autores principales: PURNOMO EDI, Suryo, IBRAHIM, Afif, SUKOCO, Rinto, BUNALI, Lukman, TAGUCHI, Masaji, KATO, Tomoko, YANASE, Tohru, SHIRAFUJI, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Virology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402201/
https://www.ncbi.nlm.nih.gov/pubmed/28302930
http://dx.doi.org/10.1292/jvms.17-0009
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author PURNOMO EDI, Suryo
IBRAHIM, Afif
SUKOCO, Rinto
BUNALI, Lukman
TAGUCHI, Masaji
KATO, Tomoko
YANASE, Tohru
SHIRAFUJI, Hiroaki
author_facet PURNOMO EDI, Suryo
IBRAHIM, Afif
SUKOCO, Rinto
BUNALI, Lukman
TAGUCHI, Masaji
KATO, Tomoko
YANASE, Tohru
SHIRAFUJI, Hiroaki
author_sort PURNOMO EDI, Suryo
collection PubMed
description We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia.
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spelling pubmed-54022012017-04-27 Molecular characterization of an Akabane virus isolate from West Java, Indonesia PURNOMO EDI, Suryo IBRAHIM, Afif SUKOCO, Rinto BUNALI, Lukman TAGUCHI, Masaji KATO, Tomoko YANASE, Tohru SHIRAFUJI, Hiroaki J Vet Med Sci Virology We isolated an arbovirus from bovine blood in Indonesia. The arbovirus was obtained from the plasma of a cow showing no clinical symptoms in West Java in February 2014, and was identified as Akabane virus (AKAV) by AKAV-specific RT-PCR and subsequent sequence analysis. Phylogenetic analysis based on partial S segment indicated the AKAV isolate, WJ-1SA/P/2014, was most closely related with two isolates from Israel and Turkey reported in 2001 and 2015, respectively, and that WJ-1SA/P/2014 isolate belongs to AKAV genogroup Ib. This is the first isolation of AKAV from Indonesia. The Japanese Society of Veterinary Science 2017-03-08 2017-04 /pmc/articles/PMC5402201/ /pubmed/28302930 http://dx.doi.org/10.1292/jvms.17-0009 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Virology
PURNOMO EDI, Suryo
IBRAHIM, Afif
SUKOCO, Rinto
BUNALI, Lukman
TAGUCHI, Masaji
KATO, Tomoko
YANASE, Tohru
SHIRAFUJI, Hiroaki
Molecular characterization of an Akabane virus isolate from West Java, Indonesia
title Molecular characterization of an Akabane virus isolate from West Java, Indonesia
title_full Molecular characterization of an Akabane virus isolate from West Java, Indonesia
title_fullStr Molecular characterization of an Akabane virus isolate from West Java, Indonesia
title_full_unstemmed Molecular characterization of an Akabane virus isolate from West Java, Indonesia
title_short Molecular characterization of an Akabane virus isolate from West Java, Indonesia
title_sort molecular characterization of an akabane virus isolate from west java, indonesia
topic Virology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402201/
https://www.ncbi.nlm.nih.gov/pubmed/28302930
http://dx.doi.org/10.1292/jvms.17-0009
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