Cargando…

Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study

Anesthetics utilized for the immobilization of pregnant mammals are prone to crossing the placental barrier and cause adverse effects to the fetuses. In this study, we develop a facile method employing high performance liquid chromatography (HPLC) for the study of Telazol crossing the placental barr...

Descripción completa

Detalles Bibliográficos
Autores principales: LI, Wei, LI, Gangshi, ZHONG, Zhijun, XIE, Bingbing, ZHOU, Ziyao, GU, Wuyang, SHI, Xianpeng, TANG, Tianliang, AI, Shengquan, FU, Hualin, LIU, Mengjiao, LIU, Mengxi, WU, De, HU, Yanchun, PENG, Guangneng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402205/
https://www.ncbi.nlm.nih.gov/pubmed/28302956
http://dx.doi.org/10.1292/jvms.16-0300
_version_ 1783231182940405760
author LI, Wei
LI, Gangshi
ZHONG, Zhijun
XIE, Bingbing
ZHOU, Ziyao
GU, Wuyang
SHI, Xianpeng
TANG, Tianliang
AI, Shengquan
FU, Hualin
LIU, Mengjiao
LIU, Mengxi
WU, De
HU, Yanchun
PENG, Guangneng
author_facet LI, Wei
LI, Gangshi
ZHONG, Zhijun
XIE, Bingbing
ZHOU, Ziyao
GU, Wuyang
SHI, Xianpeng
TANG, Tianliang
AI, Shengquan
FU, Hualin
LIU, Mengjiao
LIU, Mengxi
WU, De
HU, Yanchun
PENG, Guangneng
author_sort LI, Wei
collection PubMed
description Anesthetics utilized for the immobilization of pregnant mammals are prone to crossing the placental barrier and cause adverse effects to the fetuses. In this study, we develop a facile method employing high performance liquid chromatography (HPLC) for the study of Telazol crossing the placental barrier of pregnant pigs. The method mainly relies on the efficient extraction strategy that includes the mobile phase composed of 10 mM ammonium acetate aqueous solution-acetonitrile (1:4, v/v). When the injected dose of Telazol is 10 mg/kg (5 mg/kg of each constituent drug, zolazepem and tiletamine), zolazepam can cross the placental barrier as it is detected in both uterus and umbilical cord with approximately the same content. Conversely, tiletamine is detected in neither uterus nor umbilical cord, indicating the absence of placental transfer of tiletamine. The different absorption rates of the two dosage-equal compounds by pigs are found to be the main cause of their different abilities to cross the placental barrier.
format Online
Article
Text
id pubmed-5402205
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-54022052017-04-27 Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study LI, Wei LI, Gangshi ZHONG, Zhijun XIE, Bingbing ZHOU, Ziyao GU, Wuyang SHI, Xianpeng TANG, Tianliang AI, Shengquan FU, Hualin LIU, Mengjiao LIU, Mengxi WU, De HU, Yanchun PENG, Guangneng J Vet Med Sci Laboratory Animal Science Anesthetics utilized for the immobilization of pregnant mammals are prone to crossing the placental barrier and cause adverse effects to the fetuses. In this study, we develop a facile method employing high performance liquid chromatography (HPLC) for the study of Telazol crossing the placental barrier of pregnant pigs. The method mainly relies on the efficient extraction strategy that includes the mobile phase composed of 10 mM ammonium acetate aqueous solution-acetonitrile (1:4, v/v). When the injected dose of Telazol is 10 mg/kg (5 mg/kg of each constituent drug, zolazepem and tiletamine), zolazepam can cross the placental barrier as it is detected in both uterus and umbilical cord with approximately the same content. Conversely, tiletamine is detected in neither uterus nor umbilical cord, indicating the absence of placental transfer of tiletamine. The different absorption rates of the two dosage-equal compounds by pigs are found to be the main cause of their different abilities to cross the placental barrier. The Japanese Society of Veterinary Science 2017-03-07 2017-04 /pmc/articles/PMC5402205/ /pubmed/28302956 http://dx.doi.org/10.1292/jvms.16-0300 Text en ©2017 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Laboratory Animal Science
LI, Wei
LI, Gangshi
ZHONG, Zhijun
XIE, Bingbing
ZHOU, Ziyao
GU, Wuyang
SHI, Xianpeng
TANG, Tianliang
AI, Shengquan
FU, Hualin
LIU, Mengjiao
LIU, Mengxi
WU, De
HU, Yanchun
PENG, Guangneng
Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study
title Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study
title_full Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study
title_fullStr Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study
title_full_unstemmed Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study
title_short Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study
title_sort validation of an hplc assay for determination of telazol in pregnant pigs: application to placental transfer study
topic Laboratory Animal Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402205/
https://www.ncbi.nlm.nih.gov/pubmed/28302956
http://dx.doi.org/10.1292/jvms.16-0300
work_keys_str_mv AT liwei validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT ligangshi validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT zhongzhijun validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT xiebingbing validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT zhouziyao validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT guwuyang validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT shixianpeng validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT tangtianliang validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT aishengquan validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT fuhualin validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT liumengjiao validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT liumengxi validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT wude validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT huyanchun validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy
AT pengguangneng validationofanhplcassayfordeterminationoftelazolinpregnantpigsapplicationtoplacentaltransferstudy