Cargando…

Development of a fluorescent ASFV strain that retains the ability to cause disease in swine

African swine fever is a contagious and often lethal disease for domestic pigs with a significant economic impact for the swine industry. The etiological agent, African swine fever virus (ASFV), is a highly structurally complex double stranded DNA virus. No effective vaccines or antiviral treatment...

Descripción completa

Detalles Bibliográficos
Autores principales: Borca, Manuel V., O’Donnell, Vivian, Holinka, Lauren G., Sanford, Brent, Azzinaro, Paul A., Risatti, Guillermo R., Gladue, Douglas P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402257/
https://www.ncbi.nlm.nih.gov/pubmed/28436458
http://dx.doi.org/10.1038/srep46747
_version_ 1783231191931944960
author Borca, Manuel V.
O’Donnell, Vivian
Holinka, Lauren G.
Sanford, Brent
Azzinaro, Paul A.
Risatti, Guillermo R.
Gladue, Douglas P.
author_facet Borca, Manuel V.
O’Donnell, Vivian
Holinka, Lauren G.
Sanford, Brent
Azzinaro, Paul A.
Risatti, Guillermo R.
Gladue, Douglas P.
author_sort Borca, Manuel V.
collection PubMed
description African swine fever is a contagious and often lethal disease for domestic pigs with a significant economic impact for the swine industry. The etiological agent, African swine fever virus (ASFV), is a highly structurally complex double stranded DNA virus. No effective vaccines or antiviral treatment are currently commercially available. We present here the development of a strain of ASFV that has been shown to retain its ability to cause disease in swine, efficiently replicate in swine macrophage and that is fluorescently tagged. The insertion of an EGFP cassette replacing the reading frames for two neighboring genes, MGF360-13L and MGF360-14L, in highly virulent field isolate Georgia/2007, did not affect virus replication in cell cultures and did not affect disease progression in swine, the natural host for ASFV. A virulent fluorescently tagged ASFV is a suitable tool to conduct pathogenesis studies in swine, study on virus-macrophage interaction and to run large scale screens that require a sensitive high throughput output. Utilizing an EGFP reporter system for observing ASFV replication and infectivity can circumvent the time and labor-intensive steps associated with viral antigen-based assays such as the observation of hemadsorption or cytopathic effect.
format Online
Article
Text
id pubmed-5402257
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-54022572017-04-26 Development of a fluorescent ASFV strain that retains the ability to cause disease in swine Borca, Manuel V. O’Donnell, Vivian Holinka, Lauren G. Sanford, Brent Azzinaro, Paul A. Risatti, Guillermo R. Gladue, Douglas P. Sci Rep Article African swine fever is a contagious and often lethal disease for domestic pigs with a significant economic impact for the swine industry. The etiological agent, African swine fever virus (ASFV), is a highly structurally complex double stranded DNA virus. No effective vaccines or antiviral treatment are currently commercially available. We present here the development of a strain of ASFV that has been shown to retain its ability to cause disease in swine, efficiently replicate in swine macrophage and that is fluorescently tagged. The insertion of an EGFP cassette replacing the reading frames for two neighboring genes, MGF360-13L and MGF360-14L, in highly virulent field isolate Georgia/2007, did not affect virus replication in cell cultures and did not affect disease progression in swine, the natural host for ASFV. A virulent fluorescently tagged ASFV is a suitable tool to conduct pathogenesis studies in swine, study on virus-macrophage interaction and to run large scale screens that require a sensitive high throughput output. Utilizing an EGFP reporter system for observing ASFV replication and infectivity can circumvent the time and labor-intensive steps associated with viral antigen-based assays such as the observation of hemadsorption or cytopathic effect. Nature Publishing Group 2017-04-24 /pmc/articles/PMC5402257/ /pubmed/28436458 http://dx.doi.org/10.1038/srep46747 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Borca, Manuel V.
O’Donnell, Vivian
Holinka, Lauren G.
Sanford, Brent
Azzinaro, Paul A.
Risatti, Guillermo R.
Gladue, Douglas P.
Development of a fluorescent ASFV strain that retains the ability to cause disease in swine
title Development of a fluorescent ASFV strain that retains the ability to cause disease in swine
title_full Development of a fluorescent ASFV strain that retains the ability to cause disease in swine
title_fullStr Development of a fluorescent ASFV strain that retains the ability to cause disease in swine
title_full_unstemmed Development of a fluorescent ASFV strain that retains the ability to cause disease in swine
title_short Development of a fluorescent ASFV strain that retains the ability to cause disease in swine
title_sort development of a fluorescent asfv strain that retains the ability to cause disease in swine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402257/
https://www.ncbi.nlm.nih.gov/pubmed/28436458
http://dx.doi.org/10.1038/srep46747
work_keys_str_mv AT borcamanuelv developmentofafluorescentasfvstrainthatretainstheabilitytocausediseaseinswine
AT odonnellvivian developmentofafluorescentasfvstrainthatretainstheabilitytocausediseaseinswine
AT holinkalaureng developmentofafluorescentasfvstrainthatretainstheabilitytocausediseaseinswine
AT sanfordbrent developmentofafluorescentasfvstrainthatretainstheabilitytocausediseaseinswine
AT azzinaropaula developmentofafluorescentasfvstrainthatretainstheabilitytocausediseaseinswine
AT risattiguillermor developmentofafluorescentasfvstrainthatretainstheabilitytocausediseaseinswine
AT gladuedouglasp developmentofafluorescentasfvstrainthatretainstheabilitytocausediseaseinswine