Cargando…

Fasciola hepatica glycoconjugates immuneregulate dendritic cells through the Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin inducing T cell anergy

Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) expressed on a variety of DCs, is a C-type lectin receptor that recognizes glycans on a diverse range of pathogens, including parasites. The interaction of DC-SIGN with pathogens triggers specific signaling events that modulate DC-matura...

Descripción completa

Detalles Bibliográficos
Autores principales: Rodríguez, Ernesto, Kalay, Hakan, Noya, Verónica, Brossard, Natalie, Giacomini, Cecilia, van Kooyk, Yvette, García-Vallejo, Juan J., Freire, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402274/
https://www.ncbi.nlm.nih.gov/pubmed/28436457
http://dx.doi.org/10.1038/srep46748
_version_ 1783231196105277440
author Rodríguez, Ernesto
Kalay, Hakan
Noya, Verónica
Brossard, Natalie
Giacomini, Cecilia
van Kooyk, Yvette
García-Vallejo, Juan J.
Freire, Teresa
author_facet Rodríguez, Ernesto
Kalay, Hakan
Noya, Verónica
Brossard, Natalie
Giacomini, Cecilia
van Kooyk, Yvette
García-Vallejo, Juan J.
Freire, Teresa
author_sort Rodríguez, Ernesto
collection PubMed
description Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) expressed on a variety of DCs, is a C-type lectin receptor that recognizes glycans on a diverse range of pathogens, including parasites. The interaction of DC-SIGN with pathogens triggers specific signaling events that modulate DC-maturation and activity and regulate T-cell activation by DCs. In this work we evaluate whether F. hepatica glycans can immune modulate DCs via DC-SIGN. We demonstrate that DC-SIGN interacts with F. hepatica glycoconjugates through mannose and fucose residues. We also show that mannose is present in high-mannose structures, hybrid and trimannosyl N-glycans with terminal GlcNAc. Furthermore, we demonstrate that F. hepatica glycans induce DC-SIGN triggering leading to a strong production of TLR-induced IL-10 and IL-27p28. In addition, parasite glycans induced regulatory DCs via DC-SIGN that decrease allogeneic T cell proliferation, via the induction of anergic/regulatory T cells, highlighting the role of DC-SIGN in the regulation of innate and adaptive immune responses by F. hepatica. Our data confirm the immunomodulatory properties of DC-SIGN triggered by pathogen-derived glycans and contribute to the identification of immunomodulatory glyans of helminths that might eventually be useful for the design of vaccines against fasciolosis.
format Online
Article
Text
id pubmed-5402274
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-54022742017-04-26 Fasciola hepatica glycoconjugates immuneregulate dendritic cells through the Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin inducing T cell anergy Rodríguez, Ernesto Kalay, Hakan Noya, Verónica Brossard, Natalie Giacomini, Cecilia van Kooyk, Yvette García-Vallejo, Juan J. Freire, Teresa Sci Rep Article Dendritic cell-specific ICAM-3 grabbing non-integrin (DC-SIGN) expressed on a variety of DCs, is a C-type lectin receptor that recognizes glycans on a diverse range of pathogens, including parasites. The interaction of DC-SIGN with pathogens triggers specific signaling events that modulate DC-maturation and activity and regulate T-cell activation by DCs. In this work we evaluate whether F. hepatica glycans can immune modulate DCs via DC-SIGN. We demonstrate that DC-SIGN interacts with F. hepatica glycoconjugates through mannose and fucose residues. We also show that mannose is present in high-mannose structures, hybrid and trimannosyl N-glycans with terminal GlcNAc. Furthermore, we demonstrate that F. hepatica glycans induce DC-SIGN triggering leading to a strong production of TLR-induced IL-10 and IL-27p28. In addition, parasite glycans induced regulatory DCs via DC-SIGN that decrease allogeneic T cell proliferation, via the induction of anergic/regulatory T cells, highlighting the role of DC-SIGN in the regulation of innate and adaptive immune responses by F. hepatica. Our data confirm the immunomodulatory properties of DC-SIGN triggered by pathogen-derived glycans and contribute to the identification of immunomodulatory glyans of helminths that might eventually be useful for the design of vaccines against fasciolosis. Nature Publishing Group 2017-04-24 /pmc/articles/PMC5402274/ /pubmed/28436457 http://dx.doi.org/10.1038/srep46748 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Rodríguez, Ernesto
Kalay, Hakan
Noya, Verónica
Brossard, Natalie
Giacomini, Cecilia
van Kooyk, Yvette
García-Vallejo, Juan J.
Freire, Teresa
Fasciola hepatica glycoconjugates immuneregulate dendritic cells through the Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin inducing T cell anergy
title Fasciola hepatica glycoconjugates immuneregulate dendritic cells through the Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin inducing T cell anergy
title_full Fasciola hepatica glycoconjugates immuneregulate dendritic cells through the Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin inducing T cell anergy
title_fullStr Fasciola hepatica glycoconjugates immuneregulate dendritic cells through the Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin inducing T cell anergy
title_full_unstemmed Fasciola hepatica glycoconjugates immuneregulate dendritic cells through the Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin inducing T cell anergy
title_short Fasciola hepatica glycoconjugates immuneregulate dendritic cells through the Dendritic Cell-Specific Intercellular adhesion molecule-3-Grabbing Non-integrin inducing T cell anergy
title_sort fasciola hepatica glycoconjugates immuneregulate dendritic cells through the dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin inducing t cell anergy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402274/
https://www.ncbi.nlm.nih.gov/pubmed/28436457
http://dx.doi.org/10.1038/srep46748
work_keys_str_mv AT rodriguezernesto fasciolahepaticaglycoconjugatesimmuneregulatedendriticcellsthroughthedendriticcellspecificintercellularadhesionmolecule3grabbingnonintegrininducingtcellanergy
AT kalayhakan fasciolahepaticaglycoconjugatesimmuneregulatedendriticcellsthroughthedendriticcellspecificintercellularadhesionmolecule3grabbingnonintegrininducingtcellanergy
AT noyaveronica fasciolahepaticaglycoconjugatesimmuneregulatedendriticcellsthroughthedendriticcellspecificintercellularadhesionmolecule3grabbingnonintegrininducingtcellanergy
AT brossardnatalie fasciolahepaticaglycoconjugatesimmuneregulatedendriticcellsthroughthedendriticcellspecificintercellularadhesionmolecule3grabbingnonintegrininducingtcellanergy
AT giacominicecilia fasciolahepaticaglycoconjugatesimmuneregulatedendriticcellsthroughthedendriticcellspecificintercellularadhesionmolecule3grabbingnonintegrininducingtcellanergy
AT vankooykyvette fasciolahepaticaglycoconjugatesimmuneregulatedendriticcellsthroughthedendriticcellspecificintercellularadhesionmolecule3grabbingnonintegrininducingtcellanergy
AT garciavallejojuanj fasciolahepaticaglycoconjugatesimmuneregulatedendriticcellsthroughthedendriticcellspecificintercellularadhesionmolecule3grabbingnonintegrininducingtcellanergy
AT freireteresa fasciolahepaticaglycoconjugatesimmuneregulatedendriticcellsthroughthedendriticcellspecificintercellularadhesionmolecule3grabbingnonintegrininducingtcellanergy