Cargando…

A Novel Method to Evaluate Ribosomal Performance in Cell-Free Protein Synthesis Systems

Cell-free protein synthesis (CFPS) systems were designed to produce proteins with a minimal set of purified components, thus offering the possibility to follow translation as well as protein folding. In order to characterize the performance of the ribosomes in such a system, it is crucial to separat...

Descripción completa

Detalles Bibliográficos
Autores principales:	Kempf, Noémie, Remes, Cristina, Ledesch, Ralph, Züchner, Tina, Höfig, Henning, Ritter, Ilona, Katranidis, Alexandros, Fitter, Jörg
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2017
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402277/ https://www.ncbi.nlm.nih.gov/pubmed/28436469 http://dx.doi.org/10.1038/srep46753

Ejemplares similares

Brightness-gated two-color coincidence detection unravels two distinct mechanisms in bacterial protein translation initiation
por: Höfig, Henning, et al.
Publicado: (2019)

Impact of Molecule Concentration, Diffusion Rates and Surface Passivation on Single-Molecule Fluorescence Studies in Solution
por: Yukhnovets, Olessya, et al.
Publicado: (2022)

Single-Molecule Studies on a FRET Biosensor: Lessons from a Comparison of Fluorescent Protein Equipped versus Dye-Labeled Species
por: Höfig, Henning, et al.
Publicado: (2018)

Inter-Dye Distance Distributions Studied by a Combination of Single-Molecule FRET-Filtered Lifetime Measurements and a Weighted Accessible Volume (wAV) Algorithm
por: Höfig, Henning, et al.
Publicado: (2014)

The ribosome modulates folding inside the ribosomal exit tunnel
por: Wruck, Florian, et al.
Publicado: (2021)

Structural Analysis of a Genetically Encoded FRET Biosensor by SAXS and MD Simulations
por: Reinartz, Ines, et al.
Publicado: (2021)

Mapping Multiple Distances in a Multidomain Protein for the Identification of Folding Intermediates
por: Cerminara, Michele, et al.
Publicado: (2020)

Development of a Cell-Free Optical Biosensor for Detection of a Broad Range of Mercury Contaminants in Water: A Plasmid DNA-Based Approach
por: Gupta, Saurabh, et al.
Publicado: (2019)

Distinct pre-initiation steps in human mitochondrial translation
por: Khawaja, Anas, et al.
Publicado: (2020)

Sensitivitätsstudie zum Nachweis schwerer ungeladener Eichbosonen mit dem CMS-Detektor mit Hilfe des MUSiC-Frameworks
por: Ledesch, Sena
Publicado: (2017)

Synthesis of rat liver microsomal cytochrome b5 by free ribosomes
Publicado: (1980)

Ribosomes and protein synthesis
por: Siekevitz, P., et al.
Publicado: (1981)

In vitro ribosome synthesis and evolution through ribosome display
por: Hammerling, Michael J., et al.
Publicado: (2020)

In-Situ Observation of Membrane Protein Folding during Cell-Free Expression
por: Baumann, Axel, et al.
Publicado: (2016)

Membrane-bound ribosomes of myeloma cells. I. Preparation of free and membrane-bound ribosomal fractions. Assessment of the methods and properties of the ribosomes
Publicado: (1975)

In vitro integration of ribosomal RNA synthesis, ribosome assembly, and translation
por: Jewett, Michael C, et al.
Publicado: (2013)

RIBOSOME SYNTHESIS IN TETRAHYMENA PYRIFORMIS
por: Kumar, Ajit
Publicado: (1970)

Features of Protein Unfolding Transitions and Their Relation to Domain Topology Probed by Single-Molecule FRET
por: Bustorff, Nuno, et al.
Publicado: (2023)

LARP1 senses free ribosomes to coordinate supply and demand of ribosomal proteins
por: Saba, James A., et al.
Publicado: (2023)

Aza-Reversine Promotes Reprogramming of Lung (MRC-5) and Differentiation of Mesenchymal Cells into Osteoblasts
por: Tsitouroudi, Fani, et al.
Publicado: (2021)

Label-free electrical quantification of the dielectrophoretic response of DNA
por: Henning, Anja, et al.
Publicado: (2008)

Functional dichotomy of ribosomal proteins during the synthesis of mammalian 40S ribosomal subunits
por: O’Donohue, Marie-Françoise, et al.
Publicado: (2010)

Site-Specific Cleavage of Ribosomal RNA in Escherichia coli-Based Cell-Free Protein Synthesis Systems
por: Failmezger, Jurek, et al.
Publicado: (2016)

Ribosomal Synthesis of N-Methyl Peptides
por: Subtelny, Alexander O., et al.
Publicado: (2008)

SpAD Biofunctionalized Cellulose Acetate Scaffolds Inhibit Staphylococcus aureus Adherence in a Coordinating Function with the von Willebrand A1 Domain (vWF A1)
por: Pendas, Stefanos, et al.
Publicado: (2022)

Functional analysis of Saccharomyces cerevisiae ribosomal protein Rpl3p in ribosome synthesis
por: Rosado, Iván V., et al.
Publicado: (2007)

Neutron Scattering in Biology: Techniques and Applications
por: Fitter, Jörg, et al.
Publicado: (2006)

Ribosomal RNA Synthesis in Chronic Lymphocytic Leukaemia
por: Billington, R. W., et al.
Publicado: (1974)

Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas
Publicado: (1983)

Synthesis and degradation of ribosomal RNA in regenerating liver
Publicado: (1975)

Synthesis, antibacterial action, and ribosome inhibition of deoxyspectinomycins
por: Dharuman, Suresh, et al.
Publicado: (2021)

Membrane-bound ribosomes of myeloma cells. II. Kinetic studies on the entry of newly made ribosomal subunits into the free and the membrane- bound ribosomal particles
Publicado: (1975)

A DEAD-box protein regulates ribosome assembly through control of ribosomal protein synthesis
por: Iost, Isabelle, et al.
Publicado: (2019)

The Reverse Side of a Coin: “Factor-Free” Ribosomal Protein Synthesis In Vitro is a Consequence of the In Vivo Proofreading Mechanism
por: Finkelstein, Alexei V.
Publicado: (2019)

Prp43 Bound at Different Sites on the Pre-rRNA Performs Distinct Functions in Ribosome Synthesis
por: Bohnsack, Markus T., et al.
Publicado: (2009)

Membrane-bound ribosomes of myeloma cells. VI. Initiation of immunoglobulin mRNA translation occurs on free ribosomes
Publicado: (1981)

Boundary-Free Ribosome Compartmentalization by Gene Expression on a Surface
por: Levy, Michael, et al.
Publicado: (2021)

Ribosome Stalling of N-Linked Glycoproteins in Cell-Free Extracts
por: Chung, Sean S., et al.
Publicado: (2022)

Ribosomal History Reveals Origins of Modern Protein Synthesis
por: Harish, Ajith, et al.
Publicado: (2012)

Chaos and Hyperchaos in a Model of Ribosome Autocatalytic Synthesis
por: Likhoshvai, Vitaly A., et al.
Publicado: (2016)

Cannot write session to /tmp/vufind_sessions/sess_0kb1go7eilaopfkqjip2j6orhn