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Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity

To explore dendritic cells/tumor-derived endothelial cells (DC/EC) fusion cells are potent stimulators of T cells to impact tumor progression. ECs were isolated from mice hepatoma cell line (H22) Xenograft, and dendritic cells were isolated from bone marrow of BALB/c mice, then the isolated ECs were...

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Autores principales: Huang, Yingying, Mao, Qiqi, He, Jian, Su, Jing, Peng, Yi, Liang, Wei, Hu, Zixi, Zhou, Sufang, Lu, Xiaoling, Zhao, Yongxiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402293/
https://www.ncbi.nlm.nih.gov/pubmed/28436481
http://dx.doi.org/10.1038/srep46544
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author Huang, Yingying
Mao, Qiqi
He, Jian
Su, Jing
Peng, Yi
Liang, Wei
Hu, Zixi
Zhou, Sufang
Lu, Xiaoling
Zhao, Yongxiang
author_facet Huang, Yingying
Mao, Qiqi
He, Jian
Su, Jing
Peng, Yi
Liang, Wei
Hu, Zixi
Zhou, Sufang
Lu, Xiaoling
Zhao, Yongxiang
author_sort Huang, Yingying
collection PubMed
description To explore dendritic cells/tumor-derived endothelial cells (DC/EC) fusion cells are potent stimulators of T cells to impact tumor progression. ECs were isolated from mice hepatoma cell line (H22) Xenograft, and dendritic cells were isolated from bone marrow of BALB/c mice, then the isolated ECs were cultured and detected the endothelial surface expression of CD105 by flow cytometry. The endothelial characteristics of ECs were detected by tube formation assay and Dil-Ac-LDL uptake assay. After the fusion with polyethylene glycol (PEG), we used DCs, ECs, DCs mixed ECs as the control groups, DC/EC fusion cells as the experimental group, Secretion of IFN-α and IFN-γ was evaluated, T lymphocyte proliferation and cytotoxic T lymphocytes (CTL) were detected in vitro. In vivo, T lymphocyte induced by five groups was injected to detect the effect of tumor progression. Purified ECs (CD105(+)) took the function of endothelial cells, then successfully fused with DCs. The DC/EC fusion cells were functional in stimulating the proliferation of T cells, which produced IFN-α and IFN-γ. In vivo, T cells stimulated by DC/EC fusion cells effectively repressed tumor growth. The fusion cells, which was capable of stimulating T cells, is indispensable for antitumor immunity.
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spelling pubmed-54022932017-04-26 Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity Huang, Yingying Mao, Qiqi He, Jian Su, Jing Peng, Yi Liang, Wei Hu, Zixi Zhou, Sufang Lu, Xiaoling Zhao, Yongxiang Sci Rep Article To explore dendritic cells/tumor-derived endothelial cells (DC/EC) fusion cells are potent stimulators of T cells to impact tumor progression. ECs were isolated from mice hepatoma cell line (H22) Xenograft, and dendritic cells were isolated from bone marrow of BALB/c mice, then the isolated ECs were cultured and detected the endothelial surface expression of CD105 by flow cytometry. The endothelial characteristics of ECs were detected by tube formation assay and Dil-Ac-LDL uptake assay. After the fusion with polyethylene glycol (PEG), we used DCs, ECs, DCs mixed ECs as the control groups, DC/EC fusion cells as the experimental group, Secretion of IFN-α and IFN-γ was evaluated, T lymphocyte proliferation and cytotoxic T lymphocytes (CTL) were detected in vitro. In vivo, T lymphocyte induced by five groups was injected to detect the effect of tumor progression. Purified ECs (CD105(+)) took the function of endothelial cells, then successfully fused with DCs. The DC/EC fusion cells were functional in stimulating the proliferation of T cells, which produced IFN-α and IFN-γ. In vivo, T cells stimulated by DC/EC fusion cells effectively repressed tumor growth. The fusion cells, which was capable of stimulating T cells, is indispensable for antitumor immunity. Nature Publishing Group 2017-04-24 /pmc/articles/PMC5402293/ /pubmed/28436481 http://dx.doi.org/10.1038/srep46544 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Huang, Yingying
Mao, Qiqi
He, Jian
Su, Jing
Peng, Yi
Liang, Wei
Hu, Zixi
Zhou, Sufang
Lu, Xiaoling
Zhao, Yongxiang
Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity
title Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity
title_full Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity
title_fullStr Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity
title_full_unstemmed Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity
title_short Fusions of Tumor-derived Endothelial Cells with Dendritic Cells Induces Antitumor Immunity
title_sort fusions of tumor-derived endothelial cells with dendritic cells induces antitumor immunity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402293/
https://www.ncbi.nlm.nih.gov/pubmed/28436481
http://dx.doi.org/10.1038/srep46544
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