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Reproducibility of African giant pouched rats detecting Mycobacterium tuberculosis

BACKGROUND: African pouched rats sniffing sputum samples provided by local clinics have significantly increased tuberculosis case findings in Tanzania and Mozambique. The objective of this study was to determine the reproducibility of rat results. METHODS: Over an 18-month period 11,869 samples were...

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Autores principales: Ellis, Haylee, Mulder, Christiaan, Valverde, Emilio, Poling, Alan, Edwards, Timothy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402322/
https://www.ncbi.nlm.nih.gov/pubmed/28438117
http://dx.doi.org/10.1186/s12879-017-2347-3
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author Ellis, Haylee
Mulder, Christiaan
Valverde, Emilio
Poling, Alan
Edwards, Timothy
author_facet Ellis, Haylee
Mulder, Christiaan
Valverde, Emilio
Poling, Alan
Edwards, Timothy
author_sort Ellis, Haylee
collection PubMed
description BACKGROUND: African pouched rats sniffing sputum samples provided by local clinics have significantly increased tuberculosis case findings in Tanzania and Mozambique. The objective of this study was to determine the reproducibility of rat results. METHODS: Over an 18-month period 11,869 samples were examined by the rats. Intra-rater reliability was assessed through Yule’s Q. Inter-rater reliability was assessed with Krippendorff’s alpha. RESULTS: Intra-rater reliability was high, with a mean Yule’s Q of 0.9. Inter-rater agreement was fair, with Krippendorf’s alpha ranging from 0.15 to 0.45. Both Intra- and Inter-rater reliability was independent of the sex of the animals, but they were positively correlated with age. Both intra- and inter-rater agreement was lowest for samples designated as smear-negative by the clinics. CONCLUSION: Overall, the reproducibility of tuberculosis detection rat results was fair and diagnostic results were therefore independent of the rats used. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-017-2347-3) contains supplementary material, which is available to authorized users.
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spelling pubmed-54023222017-04-24 Reproducibility of African giant pouched rats detecting Mycobacterium tuberculosis Ellis, Haylee Mulder, Christiaan Valverde, Emilio Poling, Alan Edwards, Timothy BMC Infect Dis Research Article BACKGROUND: African pouched rats sniffing sputum samples provided by local clinics have significantly increased tuberculosis case findings in Tanzania and Mozambique. The objective of this study was to determine the reproducibility of rat results. METHODS: Over an 18-month period 11,869 samples were examined by the rats. Intra-rater reliability was assessed through Yule’s Q. Inter-rater reliability was assessed with Krippendorff’s alpha. RESULTS: Intra-rater reliability was high, with a mean Yule’s Q of 0.9. Inter-rater agreement was fair, with Krippendorf’s alpha ranging from 0.15 to 0.45. Both Intra- and Inter-rater reliability was independent of the sex of the animals, but they were positively correlated with age. Both intra- and inter-rater agreement was lowest for samples designated as smear-negative by the clinics. CONCLUSION: Overall, the reproducibility of tuberculosis detection rat results was fair and diagnostic results were therefore independent of the rats used. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12879-017-2347-3) contains supplementary material, which is available to authorized users. BioMed Central 2017-04-24 /pmc/articles/PMC5402322/ /pubmed/28438117 http://dx.doi.org/10.1186/s12879-017-2347-3 Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ellis, Haylee
Mulder, Christiaan
Valverde, Emilio
Poling, Alan
Edwards, Timothy
Reproducibility of African giant pouched rats detecting Mycobacterium tuberculosis
title Reproducibility of African giant pouched rats detecting Mycobacterium tuberculosis
title_full Reproducibility of African giant pouched rats detecting Mycobacterium tuberculosis
title_fullStr Reproducibility of African giant pouched rats detecting Mycobacterium tuberculosis
title_full_unstemmed Reproducibility of African giant pouched rats detecting Mycobacterium tuberculosis
title_short Reproducibility of African giant pouched rats detecting Mycobacterium tuberculosis
title_sort reproducibility of african giant pouched rats detecting mycobacterium tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402322/
https://www.ncbi.nlm.nih.gov/pubmed/28438117
http://dx.doi.org/10.1186/s12879-017-2347-3
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