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Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake

Superparamagnetic iron oxide nanoparticles (SPIONs) are promising tools for the treatment of different diseases. Their magnetic properties enable therapies involving magnetic drug targeting (MDT), hyperthermia or imaging. Depending on the intended treatment, specific characteristics of SPIONs are re...

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Autores principales: Poller, Johanna M, Zaloga, Jan, Schreiber, Eveline, Unterweger, Harald, Janko, Christina, Radon, Patricia, Eberbeck, Dietmar, Trahms, Lutz, Alexiou, Christoph, Friedrich, Ralf P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402883/
https://www.ncbi.nlm.nih.gov/pubmed/28458541
http://dx.doi.org/10.2147/IJN.S132369
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author Poller, Johanna M
Zaloga, Jan
Schreiber, Eveline
Unterweger, Harald
Janko, Christina
Radon, Patricia
Eberbeck, Dietmar
Trahms, Lutz
Alexiou, Christoph
Friedrich, Ralf P
author_facet Poller, Johanna M
Zaloga, Jan
Schreiber, Eveline
Unterweger, Harald
Janko, Christina
Radon, Patricia
Eberbeck, Dietmar
Trahms, Lutz
Alexiou, Christoph
Friedrich, Ralf P
author_sort Poller, Johanna M
collection PubMed
description Superparamagnetic iron oxide nanoparticles (SPIONs) are promising tools for the treatment of different diseases. Their magnetic properties enable therapies involving magnetic drug targeting (MDT), hyperthermia or imaging. Depending on the intended treatment, specific characteristics of SPIONs are required. While particles used for imaging should circulate for extended periods of time in the vascular system, SPIONs intended for MDT or hyperthermia should be accumulated in the target area to come into close proximity of, or to be incorporated into, specific tumor cells. In this study, we determined the impact of several accurately characterized SPION types varying in size, zeta potential and surface coating on various human breast cancer cell lines and endothelial cells to identify the most suitable particle for future breast cancer therapy. We analyzed cellular SPION uptake, magnetic properties, cell proliferation and toxicity using atomic emission spectroscopy, magnetic susceptometry, flow cytometry and microscopy. The results demonstrated that treatment with dextran-coated SPIONs (SPION(Dex)) and lauric acid-coated SPIONs (SPION(LA)) with an additional protein corona formed by human serum albumin (SPION(LA-HSA)) resulted in very moderate particle uptake and low cytotoxicity, whereas SPION(LA) had in part much stronger effects on cellular uptake and cellular toxicity. In summary, our data show significant dose-dependent and particle type-related response differences between various breast cancer and endothelial cells, indicating the utility of these particle types for distinct medical applications.
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spelling pubmed-54028832017-04-28 Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake Poller, Johanna M Zaloga, Jan Schreiber, Eveline Unterweger, Harald Janko, Christina Radon, Patricia Eberbeck, Dietmar Trahms, Lutz Alexiou, Christoph Friedrich, Ralf P Int J Nanomedicine Original Research Superparamagnetic iron oxide nanoparticles (SPIONs) are promising tools for the treatment of different diseases. Their magnetic properties enable therapies involving magnetic drug targeting (MDT), hyperthermia or imaging. Depending on the intended treatment, specific characteristics of SPIONs are required. While particles used for imaging should circulate for extended periods of time in the vascular system, SPIONs intended for MDT or hyperthermia should be accumulated in the target area to come into close proximity of, or to be incorporated into, specific tumor cells. In this study, we determined the impact of several accurately characterized SPION types varying in size, zeta potential and surface coating on various human breast cancer cell lines and endothelial cells to identify the most suitable particle for future breast cancer therapy. We analyzed cellular SPION uptake, magnetic properties, cell proliferation and toxicity using atomic emission spectroscopy, magnetic susceptometry, flow cytometry and microscopy. The results demonstrated that treatment with dextran-coated SPIONs (SPION(Dex)) and lauric acid-coated SPIONs (SPION(LA)) with an additional protein corona formed by human serum albumin (SPION(LA-HSA)) resulted in very moderate particle uptake and low cytotoxicity, whereas SPION(LA) had in part much stronger effects on cellular uptake and cellular toxicity. In summary, our data show significant dose-dependent and particle type-related response differences between various breast cancer and endothelial cells, indicating the utility of these particle types for distinct medical applications. Dove Medical Press 2017-04-19 /pmc/articles/PMC5402883/ /pubmed/28458541 http://dx.doi.org/10.2147/IJN.S132369 Text en © 2017 Poller et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Poller, Johanna M
Zaloga, Jan
Schreiber, Eveline
Unterweger, Harald
Janko, Christina
Radon, Patricia
Eberbeck, Dietmar
Trahms, Lutz
Alexiou, Christoph
Friedrich, Ralf P
Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_full Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_fullStr Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_full_unstemmed Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_short Selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
title_sort selection of potential iron oxide nanoparticles for breast cancer treatment based on in vitro cytotoxicity and cellular uptake
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402883/
https://www.ncbi.nlm.nih.gov/pubmed/28458541
http://dx.doi.org/10.2147/IJN.S132369
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