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Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification

Accumulation of metal contaminants in soil as a result of various industrial and anthropogenic activities has reduced soil fertility significantly. Phytoextraction of metal contaminants can improve soil fertility and provide inexpensive feedstock for biorefineries. We investigated the hyperaccumulat...

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Autores principales: Dhiman, Saurabh Sudha, Zhao, Xin, Li, Jinglin, Kim, Dongwook, Kalia, Vipin C., Kim, In-Won, Kim, Jae Young, Lee, Jung-Kul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402931/
https://www.ncbi.nlm.nih.gov/pubmed/28437478
http://dx.doi.org/10.1371/journal.pone.0175845
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author Dhiman, Saurabh Sudha
Zhao, Xin
Li, Jinglin
Kim, Dongwook
Kalia, Vipin C.
Kim, In-Won
Kim, Jae Young
Lee, Jung-Kul
author_facet Dhiman, Saurabh Sudha
Zhao, Xin
Li, Jinglin
Kim, Dongwook
Kalia, Vipin C.
Kim, In-Won
Kim, Jae Young
Lee, Jung-Kul
author_sort Dhiman, Saurabh Sudha
collection PubMed
description Accumulation of metal contaminants in soil as a result of various industrial and anthropogenic activities has reduced soil fertility significantly. Phytoextraction of metal contaminants can improve soil fertility and provide inexpensive feedstock for biorefineries. We investigated the hyperaccumulation capacity of sunflower (Helianthus annuus) biomass by cultivating these plants in various concentrations of metal contaminants. Sunflowers were grown in soils contaminated with various levels of heavy metals (10–2,000 mg/kg dry soil). The degree of metal uptake by different parts of the biomass and the residual concentration in the soil were estimated through inductively coupled plasma mass spectrometry. An almost 2.5-fold hyperaccumulation of Zn(2+) was observed in the leaf and flower biomass compared with the concentration in the soil. For the subsequent saccharification of biomass with hyperaccumulated contaminants, a fungal lignocellulosic consortium was used. The fungal consortium cocktail retained more than 95% filter paper activity with 100 mM Ni(2+) ions even after 36 h. The highest saccharification yield (SY, 87.4%) was observed with Ni(2+) as the contaminant (10 mg/kg dry wt), whereas Pb(2+) (251.9 mg/kg dry wt) was the strongest inhibitor of biomass hydrolysis, resulting in only a 30% SY. Importantly, the enzyme cocktail produced by the fungal consortium resulted in almost the same SY (%) as that obtained from a combination of commercial cellulase and β-glucosidase. Significant sugar conversion (61.7%) from H. annuus biomass hydrolysate occurred, resulting in the production of 11.4 g/L of bioethanol. This is the first study to assess the suitability of phytoremediated sunflower biomass for bioethanol production.
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spelling pubmed-54029312017-05-12 Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification Dhiman, Saurabh Sudha Zhao, Xin Li, Jinglin Kim, Dongwook Kalia, Vipin C. Kim, In-Won Kim, Jae Young Lee, Jung-Kul PLoS One Research Article Accumulation of metal contaminants in soil as a result of various industrial and anthropogenic activities has reduced soil fertility significantly. Phytoextraction of metal contaminants can improve soil fertility and provide inexpensive feedstock for biorefineries. We investigated the hyperaccumulation capacity of sunflower (Helianthus annuus) biomass by cultivating these plants in various concentrations of metal contaminants. Sunflowers were grown in soils contaminated with various levels of heavy metals (10–2,000 mg/kg dry soil). The degree of metal uptake by different parts of the biomass and the residual concentration in the soil were estimated through inductively coupled plasma mass spectrometry. An almost 2.5-fold hyperaccumulation of Zn(2+) was observed in the leaf and flower biomass compared with the concentration in the soil. For the subsequent saccharification of biomass with hyperaccumulated contaminants, a fungal lignocellulosic consortium was used. The fungal consortium cocktail retained more than 95% filter paper activity with 100 mM Ni(2+) ions even after 36 h. The highest saccharification yield (SY, 87.4%) was observed with Ni(2+) as the contaminant (10 mg/kg dry wt), whereas Pb(2+) (251.9 mg/kg dry wt) was the strongest inhibitor of biomass hydrolysis, resulting in only a 30% SY. Importantly, the enzyme cocktail produced by the fungal consortium resulted in almost the same SY (%) as that obtained from a combination of commercial cellulase and β-glucosidase. Significant sugar conversion (61.7%) from H. annuus biomass hydrolysate occurred, resulting in the production of 11.4 g/L of bioethanol. This is the first study to assess the suitability of phytoremediated sunflower biomass for bioethanol production. Public Library of Science 2017-04-24 /pmc/articles/PMC5402931/ /pubmed/28437478 http://dx.doi.org/10.1371/journal.pone.0175845 Text en © 2017 Dhiman et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Dhiman, Saurabh Sudha
Zhao, Xin
Li, Jinglin
Kim, Dongwook
Kalia, Vipin C.
Kim, In-Won
Kim, Jae Young
Lee, Jung-Kul
Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification
title Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification
title_full Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification
title_fullStr Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification
title_full_unstemmed Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification
title_short Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification
title_sort metal accumulation by sunflower (helianthus annuus l.) and the efficacy of its biomass in enzymatic saccharification
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402931/
https://www.ncbi.nlm.nih.gov/pubmed/28437478
http://dx.doi.org/10.1371/journal.pone.0175845
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