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Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil

Streptomyces strains isolated from Nelliyampathy forest soil of Western Ghats, Kerala, India were evaluated for their antibacterial efficacy against two indicator pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Among 140 strains tested, sixteen recorded potent antibacterial propert...

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Autores principales: Jacob, Jubi, Rajendran, Reshma Uma, Priya, Syama Hari, Purushothaman, Jayamurthy, Saraswathy Amma, Dileep Kumar Bhaskaran Nair
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402949/
https://www.ncbi.nlm.nih.gov/pubmed/28437452
http://dx.doi.org/10.1371/journal.pone.0175919
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author Jacob, Jubi
Rajendran, Reshma Uma
Priya, Syama Hari
Purushothaman, Jayamurthy
Saraswathy Amma, Dileep Kumar Bhaskaran Nair
author_facet Jacob, Jubi
Rajendran, Reshma Uma
Priya, Syama Hari
Purushothaman, Jayamurthy
Saraswathy Amma, Dileep Kumar Bhaskaran Nair
author_sort Jacob, Jubi
collection PubMed
description Streptomyces strains isolated from Nelliyampathy forest soil of Western Ghats, Kerala, India were evaluated for their antibacterial efficacy against two indicator pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Among 140 strains tested, sixteen recorded potent antibacterial properties and were further screened against eleven bacterial pathogens. A strain identified as Streptomyces nogalater and designated as NIIST A30 exhibited maximum inhibition against all the test pathogens. Among the eight fermentation media tested, inorganic salts starch broth recorded the best for antibacterial production. The ethyl acetate crude extract exhibited antioxidant properties with IC(50) value of 30 μg/mL and had no cytotoxicity towards L6, H9c2 and RAW 264.7 cell lines up to a concentration of 50 μg/mL. Maximum metabolite production was achieved in pH 7.0 at 35°C after 7 days incubation. The significant media components for maximum metabolite production were optimized through response surface methodology employing Plackett-Burman and Box-Behnken designs. The composition of the final optimized medium was soluble starch, 14.97g; (NH(4))(2)SO(4), 2.89g; K(2)HPO(4), 2.07g; MgSO(4).7H(2)O, 1g; NaCl, 1g, CaCO(3), 2g; FeSO(4).7H(2)O, 1mg; MnCl(2).7H(2)O, 1mg; and ZnSO(4).7H(2)O, 1mg per litre of distilled water. The optimization resulted an antibacterial activity of 28±1.5mm against S. epidermidis which was in close accordance with the predicted value of 30 mm. It is also evident from the result that an increase of 86.66% antibacterial production was recorded in optimized media. The chosen method was economical, efficient and useful for future antibacterial drug discovery from a broad spectrum metabolite producer like Streptomyces nogalater NIIST A30.
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spelling pubmed-54029492017-05-12 Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil Jacob, Jubi Rajendran, Reshma Uma Priya, Syama Hari Purushothaman, Jayamurthy Saraswathy Amma, Dileep Kumar Bhaskaran Nair PLoS One Research Article Streptomyces strains isolated from Nelliyampathy forest soil of Western Ghats, Kerala, India were evaluated for their antibacterial efficacy against two indicator pathogenic bacteria (Escherichia coli and Staphylococcus aureus). Among 140 strains tested, sixteen recorded potent antibacterial properties and were further screened against eleven bacterial pathogens. A strain identified as Streptomyces nogalater and designated as NIIST A30 exhibited maximum inhibition against all the test pathogens. Among the eight fermentation media tested, inorganic salts starch broth recorded the best for antibacterial production. The ethyl acetate crude extract exhibited antioxidant properties with IC(50) value of 30 μg/mL and had no cytotoxicity towards L6, H9c2 and RAW 264.7 cell lines up to a concentration of 50 μg/mL. Maximum metabolite production was achieved in pH 7.0 at 35°C after 7 days incubation. The significant media components for maximum metabolite production were optimized through response surface methodology employing Plackett-Burman and Box-Behnken designs. The composition of the final optimized medium was soluble starch, 14.97g; (NH(4))(2)SO(4), 2.89g; K(2)HPO(4), 2.07g; MgSO(4).7H(2)O, 1g; NaCl, 1g, CaCO(3), 2g; FeSO(4).7H(2)O, 1mg; MnCl(2).7H(2)O, 1mg; and ZnSO(4).7H(2)O, 1mg per litre of distilled water. The optimization resulted an antibacterial activity of 28±1.5mm against S. epidermidis which was in close accordance with the predicted value of 30 mm. It is also evident from the result that an increase of 86.66% antibacterial production was recorded in optimized media. The chosen method was economical, efficient and useful for future antibacterial drug discovery from a broad spectrum metabolite producer like Streptomyces nogalater NIIST A30. Public Library of Science 2017-04-24 /pmc/articles/PMC5402949/ /pubmed/28437452 http://dx.doi.org/10.1371/journal.pone.0175919 Text en © 2017 Jacob et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Jacob, Jubi
Rajendran, Reshma Uma
Priya, Syama Hari
Purushothaman, Jayamurthy
Saraswathy Amma, Dileep Kumar Bhaskaran Nair
Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil
title Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil
title_full Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil
title_fullStr Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil
title_full_unstemmed Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil
title_short Enhanced antibacterial metabolite production through the application of statistical methodologies by a Streptomyces nogalater NIIST A30 isolated from Western Ghats forest soil
title_sort enhanced antibacterial metabolite production through the application of statistical methodologies by a streptomyces nogalater niist a30 isolated from western ghats forest soil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5402949/
https://www.ncbi.nlm.nih.gov/pubmed/28437452
http://dx.doi.org/10.1371/journal.pone.0175919
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