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Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors

The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation was to develop a novel biological...

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Autores principales: Kakabadze, Ann, Mardaleishvili, Konstantine, Loladze, George, Karalashvili, Lia, Chutkerashvili, Gocha, Chakhunashvili, David, Kakabadze, Zurab
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5403483/
https://www.ncbi.nlm.nih.gov/pubmed/28454328
http://dx.doi.org/10.3892/ol.2017.5647
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author Kakabadze, Ann
Mardaleishvili, Konstantine
Loladze, George
Karalashvili, Lia
Chutkerashvili, Gocha
Chakhunashvili, David
Kakabadze, Zurab
author_facet Kakabadze, Ann
Mardaleishvili, Konstantine
Loladze, George
Karalashvili, Lia
Chutkerashvili, Gocha
Chakhunashvili, David
Kakabadze, Zurab
author_sort Kakabadze, Ann
collection PubMed
description The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation was to develop a novel biologically active bone (BAB) graft, and to use it for the reconstruction of large size defects of the mandible bone following tumor resection. In the first part of the present study, biologically active bone graft was developed by using human freeze-dried bone marrow stem cells (BMSCs) paracrine factors and three-dimensional bone scaffold derived from cancellous bovine bone following decellularization. In the second part of the research, one male and three female patients with primary tumors of the mandible underwent hemimandibulectomy. The mandibular bone defects following tumor resection were reconstructed with autogenous rib grafts in three patients and BAB graft was used in one patient. The graft-host interfaces were covered with decellularized human amnion/chorion membrane graft. All patients were followed-up every five months following the reconstruction of the mandible, with no complications observed. Preliminary clinical investigations demonstrated that a BAB graft containing freeze-dried BMSC paracrine factors may be used for the reconstruction of large mandibular bone defects following tumor resection.
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spelling pubmed-54034832017-04-27 Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors Kakabadze, Ann Mardaleishvili, Konstantine Loladze, George Karalashvili, Lia Chutkerashvili, Gocha Chakhunashvili, David Kakabadze, Zurab Oncol Lett Articles The gold standard following segmental mandibulectomy is vascularized autologous bone graft in the form of the fibula flap. However, in bone reconstruction the use of autogenous bone does not always guarantee a successful outcome. The aim of the present investigation was to develop a novel biologically active bone (BAB) graft, and to use it for the reconstruction of large size defects of the mandible bone following tumor resection. In the first part of the present study, biologically active bone graft was developed by using human freeze-dried bone marrow stem cells (BMSCs) paracrine factors and three-dimensional bone scaffold derived from cancellous bovine bone following decellularization. In the second part of the research, one male and three female patients with primary tumors of the mandible underwent hemimandibulectomy. The mandibular bone defects following tumor resection were reconstructed with autogenous rib grafts in three patients and BAB graft was used in one patient. The graft-host interfaces were covered with decellularized human amnion/chorion membrane graft. All patients were followed-up every five months following the reconstruction of the mandible, with no complications observed. Preliminary clinical investigations demonstrated that a BAB graft containing freeze-dried BMSC paracrine factors may be used for the reconstruction of large mandibular bone defects following tumor resection. D.A. Spandidos 2017-03 2017-01-25 /pmc/articles/PMC5403483/ /pubmed/28454328 http://dx.doi.org/10.3892/ol.2017.5647 Text en Copyright: © Kakabadze et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Kakabadze, Ann
Mardaleishvili, Konstantine
Loladze, George
Karalashvili, Lia
Chutkerashvili, Gocha
Chakhunashvili, David
Kakabadze, Zurab
Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors
title Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors
title_full Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors
title_fullStr Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors
title_full_unstemmed Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors
title_short Reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors
title_sort reconstruction of mandibular defects with autogenous bone and decellularized bovine bone grafts with freeze-dried bone marrow stem cell paracrine factors
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5403483/
https://www.ncbi.nlm.nih.gov/pubmed/28454328
http://dx.doi.org/10.3892/ol.2017.5647
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