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A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo

Species tropism constitutes a serious problem for developing relevant animal models of infection. Human pathogens can express virulence factors that show specific selectivity to human proteins, while their affinity for orthologs from other species can vary significantly. Suitable animal species must...

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Autores principales: Naudin, Clément, Schumski, Ariane, Salo‐Ahen, Outi M. H., Herwald, Heiko, Smeds, Emanuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404189/
https://www.ncbi.nlm.nih.gov/pubmed/28168836
http://dx.doi.org/10.1111/1751-7915.12601
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author Naudin, Clément
Schumski, Ariane
Salo‐Ahen, Outi M. H.
Herwald, Heiko
Smeds, Emanuel
author_facet Naudin, Clément
Schumski, Ariane
Salo‐Ahen, Outi M. H.
Herwald, Heiko
Smeds, Emanuel
author_sort Naudin, Clément
collection PubMed
description Species tropism constitutes a serious problem for developing relevant animal models of infection. Human pathogens can express virulence factors that show specific selectivity to human proteins, while their affinity for orthologs from other species can vary significantly. Suitable animal species must be used to analyse whether virulence factors are potential targets for drug development. We developed an assay that rapidly predicts applicable animal species for studying virulence factors binding plasma proteins. We used two well‐characterized Staphylococcus aureus proteins, SSL7 and Efb, to develop an ELISA‐based inhibition assay using plasma from different animal species. The interaction between SSL7 and human C5 and the binding of Efb to human fibrinogen and human C3 was studied. Affinity experiments and Western blot analyses were used to validate the assay. Human, monkey and cat plasma interfered with binding of SSL7 to human C5. Binding of Efb to human fibrinogen was blocked in human, monkey, gerbil and pig plasma, while human, monkey, gerbil, rabbit, cat and guinea pig plasma inhibited the binding of Efb to human C3. These results emphasize the importance of choosing correct animal models, and thus, our approach is a rapid and cost‐effective method that can be used to prevent unnecessary animal experiments.
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spelling pubmed-54041892017-04-27 A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo Naudin, Clément Schumski, Ariane Salo‐Ahen, Outi M. H. Herwald, Heiko Smeds, Emanuel Microb Biotechnol Brief Report Species tropism constitutes a serious problem for developing relevant animal models of infection. Human pathogens can express virulence factors that show specific selectivity to human proteins, while their affinity for orthologs from other species can vary significantly. Suitable animal species must be used to analyse whether virulence factors are potential targets for drug development. We developed an assay that rapidly predicts applicable animal species for studying virulence factors binding plasma proteins. We used two well‐characterized Staphylococcus aureus proteins, SSL7 and Efb, to develop an ELISA‐based inhibition assay using plasma from different animal species. The interaction between SSL7 and human C5 and the binding of Efb to human fibrinogen and human C3 was studied. Affinity experiments and Western blot analyses were used to validate the assay. Human, monkey and cat plasma interfered with binding of SSL7 to human C5. Binding of Efb to human fibrinogen was blocked in human, monkey, gerbil and pig plasma, while human, monkey, gerbil, rabbit, cat and guinea pig plasma inhibited the binding of Efb to human C3. These results emphasize the importance of choosing correct animal models, and thus, our approach is a rapid and cost‐effective method that can be used to prevent unnecessary animal experiments. John Wiley and Sons Inc. 2017-02-07 /pmc/articles/PMC5404189/ /pubmed/28168836 http://dx.doi.org/10.1111/1751-7915.12601 Text en © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Report
Naudin, Clément
Schumski, Ariane
Salo‐Ahen, Outi M. H.
Herwald, Heiko
Smeds, Emanuel
A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
title A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
title_full A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
title_fullStr A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
title_full_unstemmed A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
title_short A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
title_sort rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404189/
https://www.ncbi.nlm.nih.gov/pubmed/28168836
http://dx.doi.org/10.1111/1751-7915.12601
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