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A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
Species tropism constitutes a serious problem for developing relevant animal models of infection. Human pathogens can express virulence factors that show specific selectivity to human proteins, while their affinity for orthologs from other species can vary significantly. Suitable animal species must...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404189/ https://www.ncbi.nlm.nih.gov/pubmed/28168836 http://dx.doi.org/10.1111/1751-7915.12601 |
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author | Naudin, Clément Schumski, Ariane Salo‐Ahen, Outi M. H. Herwald, Heiko Smeds, Emanuel |
author_facet | Naudin, Clément Schumski, Ariane Salo‐Ahen, Outi M. H. Herwald, Heiko Smeds, Emanuel |
author_sort | Naudin, Clément |
collection | PubMed |
description | Species tropism constitutes a serious problem for developing relevant animal models of infection. Human pathogens can express virulence factors that show specific selectivity to human proteins, while their affinity for orthologs from other species can vary significantly. Suitable animal species must be used to analyse whether virulence factors are potential targets for drug development. We developed an assay that rapidly predicts applicable animal species for studying virulence factors binding plasma proteins. We used two well‐characterized Staphylococcus aureus proteins, SSL7 and Efb, to develop an ELISA‐based inhibition assay using plasma from different animal species. The interaction between SSL7 and human C5 and the binding of Efb to human fibrinogen and human C3 was studied. Affinity experiments and Western blot analyses were used to validate the assay. Human, monkey and cat plasma interfered with binding of SSL7 to human C5. Binding of Efb to human fibrinogen was blocked in human, monkey, gerbil and pig plasma, while human, monkey, gerbil, rabbit, cat and guinea pig plasma inhibited the binding of Efb to human C3. These results emphasize the importance of choosing correct animal models, and thus, our approach is a rapid and cost‐effective method that can be used to prevent unnecessary animal experiments. |
format | Online Article Text |
id | pubmed-5404189 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54041892017-04-27 A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo Naudin, Clément Schumski, Ariane Salo‐Ahen, Outi M. H. Herwald, Heiko Smeds, Emanuel Microb Biotechnol Brief Report Species tropism constitutes a serious problem for developing relevant animal models of infection. Human pathogens can express virulence factors that show specific selectivity to human proteins, while their affinity for orthologs from other species can vary significantly. Suitable animal species must be used to analyse whether virulence factors are potential targets for drug development. We developed an assay that rapidly predicts applicable animal species for studying virulence factors binding plasma proteins. We used two well‐characterized Staphylococcus aureus proteins, SSL7 and Efb, to develop an ELISA‐based inhibition assay using plasma from different animal species. The interaction between SSL7 and human C5 and the binding of Efb to human fibrinogen and human C3 was studied. Affinity experiments and Western blot analyses were used to validate the assay. Human, monkey and cat plasma interfered with binding of SSL7 to human C5. Binding of Efb to human fibrinogen was blocked in human, monkey, gerbil and pig plasma, while human, monkey, gerbil, rabbit, cat and guinea pig plasma inhibited the binding of Efb to human C3. These results emphasize the importance of choosing correct animal models, and thus, our approach is a rapid and cost‐effective method that can be used to prevent unnecessary animal experiments. John Wiley and Sons Inc. 2017-02-07 /pmc/articles/PMC5404189/ /pubmed/28168836 http://dx.doi.org/10.1111/1751-7915.12601 Text en © 2017 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. This is an open access article under the terms of the Creative Commons Attribution (http://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Brief Report Naudin, Clément Schumski, Ariane Salo‐Ahen, Outi M. H. Herwald, Heiko Smeds, Emanuel A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo |
title | A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
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title_full | A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
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title_fullStr | A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
|
title_full_unstemmed | A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
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title_short | A rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo
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title_sort | rapid method for selecting suitable animal species for studying pathogen interactions with plasma protein ligands in vivo |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404189/ https://www.ncbi.nlm.nih.gov/pubmed/28168836 http://dx.doi.org/10.1111/1751-7915.12601 |
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