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Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases
BACKGROUND: Τo perform a molecular epidemiological analysis of viral conjunctivitis among excess conjunctivitis cases recorded at the University Hospital of Patras, Greece, for the period March to June 2012. METHODS: A structured questionnaire containing demographic and clinical data was developed i...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404675/ https://www.ncbi.nlm.nih.gov/pubmed/28438142 http://dx.doi.org/10.1186/s12886-017-0447-x |
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author | Balasopoulou, A. Κokkinos, P. Pagoulatos, D. Plotas, P. Makri, O. E. Georgakopoulos, C. D. Vantarakis, A. |
author_facet | Balasopoulou, A. Κokkinos, P. Pagoulatos, D. Plotas, P. Makri, O. E. Georgakopoulos, C. D. Vantarakis, A. |
author_sort | Balasopoulou, A. |
collection | PubMed |
description | BACKGROUND: Τo perform a molecular epidemiological analysis of viral conjunctivitis among excess conjunctivitis cases recorded at the University Hospital of Patras, Greece, for the period March to June 2012. METHODS: A structured questionnaire containing demographic and clinical data was developed in order to collect retrospective data on the cases. Eye swab specimens were collected and molecular detection of adenoviruses was performed by nested PCR. Positive results were confirmed by sequencing. To determine the relatedness between the isolated sequences, a phylogenetic analysis was conducted. RESULTS: The epidemiological analysis (including retrospective data) included 231 conjunctivitis cases (47.1% male, and 52.8% female). Based on clinical features 205 of the cases were diagnosed of viral origin (46.3% male and 53.7% female), 4 of bacterial origin (50% male and 50% female) while 22 were undefined conjunctivitis. The outbreak excess cases (included 156 cases) affected all age groups regardless gender predilection. For the positive samples indicated that 29 samples (72.5%) were AdV17, and 5 (12.5%) as AdV54. CONCLUSIONS: Molecular analysis could define the cause of viral conjunctivitis, while epidemiological data contributed to the assessment of the risk factors and underlined possible preventive measures. This study is one of the very few on viral conjunctivitis in Greece. This outbreak underscores the need for a national surveillance system for acute infectious conjunctivitis outbreaks. The epidemiological as well as molecular investigation on HAdV ocular infections is rather absent in Greece, which has no surveillance system for viral conjunctivitis. |
format | Online Article Text |
id | pubmed-5404675 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54046752017-04-27 Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases Balasopoulou, A. Κokkinos, P. Pagoulatos, D. Plotas, P. Makri, O. E. Georgakopoulos, C. D. Vantarakis, A. BMC Ophthalmol Research Article BACKGROUND: Τo perform a molecular epidemiological analysis of viral conjunctivitis among excess conjunctivitis cases recorded at the University Hospital of Patras, Greece, for the period March to June 2012. METHODS: A structured questionnaire containing demographic and clinical data was developed in order to collect retrospective data on the cases. Eye swab specimens were collected and molecular detection of adenoviruses was performed by nested PCR. Positive results were confirmed by sequencing. To determine the relatedness between the isolated sequences, a phylogenetic analysis was conducted. RESULTS: The epidemiological analysis (including retrospective data) included 231 conjunctivitis cases (47.1% male, and 52.8% female). Based on clinical features 205 of the cases were diagnosed of viral origin (46.3% male and 53.7% female), 4 of bacterial origin (50% male and 50% female) while 22 were undefined conjunctivitis. The outbreak excess cases (included 156 cases) affected all age groups regardless gender predilection. For the positive samples indicated that 29 samples (72.5%) were AdV17, and 5 (12.5%) as AdV54. CONCLUSIONS: Molecular analysis could define the cause of viral conjunctivitis, while epidemiological data contributed to the assessment of the risk factors and underlined possible preventive measures. This study is one of the very few on viral conjunctivitis in Greece. This outbreak underscores the need for a national surveillance system for acute infectious conjunctivitis outbreaks. The epidemiological as well as molecular investigation on HAdV ocular infections is rather absent in Greece, which has no surveillance system for viral conjunctivitis. BioMed Central 2017-04-24 /pmc/articles/PMC5404675/ /pubmed/28438142 http://dx.doi.org/10.1186/s12886-017-0447-x Text en © The Author(s). 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Balasopoulou, A. Κokkinos, P. Pagoulatos, D. Plotas, P. Makri, O. E. Georgakopoulos, C. D. Vantarakis, A. Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases |
title | Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases |
title_full | Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases |
title_fullStr | Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases |
title_full_unstemmed | Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases |
title_short | Α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases |
title_sort | α molecular epidemiological analysis of adenoviruses from excess conjunctivitis cases |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404675/ https://www.ncbi.nlm.nih.gov/pubmed/28438142 http://dx.doi.org/10.1186/s12886-017-0447-x |
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