Large scale validation of an efficient CRISPR/Cas-based multi gene editing protocol in Escherichia coli

BACKGROUND: The exploitation of the CRISPR/Cas9 machinery coupled to lambda (λ) recombinase-mediated homologous recombination (recombineering) is becoming the method of choice for genome editing in E. coli. First proposed by Jiang and co-workers, the strategy has been subsequently fine-tuned by seve...

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Detalles Bibliográficos
Autores principales: Zerbini, Francesca, Zanella, Ilaria, Fraccascia, Davide, König, Enrico, Irene, Carmela, Frattini, Luca F., Tomasi, Michele, Fantappiè, Laura, Ganfini, Luisa, Caproni, Elena, Parri, Matteo, Grandi, Alberto, Grandi, Guido
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404680/
https://www.ncbi.nlm.nih.gov/pubmed/28438207
http://dx.doi.org/10.1186/s12934-017-0681-1

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