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In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria
OBJECTIVE: Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of halitosis. T...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Faculdade De Odontologia De Bauru - USP
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404886/ https://www.ncbi.nlm.nih.gov/pubmed/28076463 http://dx.doi.org/10.1590/1678-775720160044 |
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author | GRAZIANO, Talita Signoreti CALIL, Caroline Morini SARTORATTO, Adilson FRANCO, Gilson César Nobre GROPPO, Francisco Carlos COGO-MÜLLER, Karina |
author_facet | GRAZIANO, Talita Signoreti CALIL, Caroline Morini SARTORATTO, Adilson FRANCO, Gilson César Nobre GROPPO, Francisco Carlos COGO-MÜLLER, Karina |
author_sort | GRAZIANO, Talita Signoreti |
collection | PubMed |
description | OBJECTIVE: Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of halitosis. The aim of this study was to evaluate the antimicrobial activity of the essential oil of Melaleuca alternifolia on the growth and volatile sulphur compound (VSC) production of oral bacteria compared with chlorhexidine. MATERIAL AND METHODS: The effects of these substances were evaluated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in planktonic cultures of Porphyromonas gingivalis and Porphyromonas endodontalis. In addition, gas chromatography analyses were performed to measure the concentration of VSCs from bacterial cultures and to characterize M. alternifolia oil components. RESULTS: The MIC and MBC values were as follows: M. alternifolia - P. gingivalis (MIC and MBC=0.007%), P. endodontalis (MIC and MBC=0.007%=0.5%); chlorhexidine - P. gingivalis and P. endodontalis (MIC and MBC=1.5 mg/mL). M. alternifolia significantly reduced the growth and production of hydrogen sulfide (H(2)S) by P. gingivalis (p<0.05, ANOVA-Dunnet) and the H(2)S and methyl mercaptan (CH(3)SH) levels of P. endodontalis (p<0.05, ANOVA-Dunnet). Chlorhexidine reduced the growth of both microorganisms without altering the production of VSC in P. endodontalis. For P. gingivalis, the production of H(2)S and CH(3)SH decreased (p<0.05, ANOVA-Dunnet). CONCLUSION: M. alternifolia can reduce bacterial growth and VSCs production and could be used as an alternative to chlorhexidine. |
format | Online Article Text |
id | pubmed-5404886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Faculdade De Odontologia De Bauru - USP |
record_format | MEDLINE/PubMed |
spelling | pubmed-54048862017-04-28 In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria GRAZIANO, Talita Signoreti CALIL, Caroline Morini SARTORATTO, Adilson FRANCO, Gilson César Nobre GROPPO, Francisco Carlos COGO-MÜLLER, Karina J Appl Oral Sci Original Articles OBJECTIVE: Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of halitosis. The aim of this study was to evaluate the antimicrobial activity of the essential oil of Melaleuca alternifolia on the growth and volatile sulphur compound (VSC) production of oral bacteria compared with chlorhexidine. MATERIAL AND METHODS: The effects of these substances were evaluated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in planktonic cultures of Porphyromonas gingivalis and Porphyromonas endodontalis. In addition, gas chromatography analyses were performed to measure the concentration of VSCs from bacterial cultures and to characterize M. alternifolia oil components. RESULTS: The MIC and MBC values were as follows: M. alternifolia - P. gingivalis (MIC and MBC=0.007%), P. endodontalis (MIC and MBC=0.007%=0.5%); chlorhexidine - P. gingivalis and P. endodontalis (MIC and MBC=1.5 mg/mL). M. alternifolia significantly reduced the growth and production of hydrogen sulfide (H(2)S) by P. gingivalis (p<0.05, ANOVA-Dunnet) and the H(2)S and methyl mercaptan (CH(3)SH) levels of P. endodontalis (p<0.05, ANOVA-Dunnet). Chlorhexidine reduced the growth of both microorganisms without altering the production of VSC in P. endodontalis. For P. gingivalis, the production of H(2)S and CH(3)SH decreased (p<0.05, ANOVA-Dunnet). CONCLUSION: M. alternifolia can reduce bacterial growth and VSCs production and could be used as an alternative to chlorhexidine. Faculdade De Odontologia De Bauru - USP 2016 /pmc/articles/PMC5404886/ /pubmed/28076463 http://dx.doi.org/10.1590/1678-775720160044 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles GRAZIANO, Talita Signoreti CALIL, Caroline Morini SARTORATTO, Adilson FRANCO, Gilson César Nobre GROPPO, Francisco Carlos COGO-MÜLLER, Karina In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
title |
In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
title_full |
In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
title_fullStr |
In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
title_full_unstemmed |
In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
title_short |
In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
title_sort | in vitro effects of melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5404886/ https://www.ncbi.nlm.nih.gov/pubmed/28076463 http://dx.doi.org/10.1590/1678-775720160044 |
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