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Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory

Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella...

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Autores principales: Xia, Xiaofeng, Gurr, Geoff M., Vasseur, Liette, Zheng, Dandan, Zhong, Huanzi, Qin, Bingcai, Lin, Junhan, Wang, Yue, Song, FengQin, Li, Yong, Lin, Hailan, You, Minsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405146/
https://www.ncbi.nlm.nih.gov/pubmed/28491055
http://dx.doi.org/10.3389/fmicb.2017.00663
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author Xia, Xiaofeng
Gurr, Geoff M.
Vasseur, Liette
Zheng, Dandan
Zhong, Huanzi
Qin, Bingcai
Lin, Junhan
Wang, Yue
Song, FengQin
Li, Yong
Lin, Hailan
You, Minsheng
author_facet Xia, Xiaofeng
Gurr, Geoff M.
Vasseur, Liette
Zheng, Dandan
Zhong, Huanzi
Qin, Bingcai
Lin, Junhan
Wang, Yue
Song, FengQin
Li, Yong
Lin, Hailan
You, Minsheng
author_sort Xia, Xiaofeng
collection PubMed
description Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. Here we expand on that work with a complete taxonomic and functional profile of the P. xylostella gut microbiota obtained by metagenomic sequencing. Gene enrichment in the metagenome, accompanied by functional identification, revealed an important role of specific gut bacteria in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids. Microbes participating in these pathways mainly belonged to three highly abundant bacteria: Enterobacter cloacae, Enterobacter asburiae, and Carnobacterium maltaromaticum. Results show that while the gut microbial community may be complex, a small number of functionally active species can be disproportionally important. The presence of specific enzymes in the microbiota community, such as supporting amino acid synthesis, digestion and detoxification functions, demonstrates the beneficial interactions between P. xylostella and its gut microbiota. These interactions can be potential targets for manipulation to provide novel pest management approaches.
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spelling pubmed-54051462017-05-10 Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory Xia, Xiaofeng Gurr, Geoff M. Vasseur, Liette Zheng, Dandan Zhong, Huanzi Qin, Bingcai Lin, Junhan Wang, Yue Song, FengQin Li, Yong Lin, Hailan You, Minsheng Front Microbiol Microbiology Herbivore specialists adapt to feed on a specific group of host plants by evolving various mechanisms to respond to plant defenses. Insects also possess complex gut microbiotas but their potential role in adaptation is poorly understood. Our previous study of the genome of diamondback moth, Plutella xylostella, revealed an intrinsic capacity to detoxify plant defense compounds, which is an important factor in its success as a pest. Here we expand on that work with a complete taxonomic and functional profile of the P. xylostella gut microbiota obtained by metagenomic sequencing. Gene enrichment in the metagenome, accompanied by functional identification, revealed an important role of specific gut bacteria in the breakdown of plant cell walls, detoxification of plant phenolics, and synthesis of amino acids. Microbes participating in these pathways mainly belonged to three highly abundant bacteria: Enterobacter cloacae, Enterobacter asburiae, and Carnobacterium maltaromaticum. Results show that while the gut microbial community may be complex, a small number of functionally active species can be disproportionally important. The presence of specific enzymes in the microbiota community, such as supporting amino acid synthesis, digestion and detoxification functions, demonstrates the beneficial interactions between P. xylostella and its gut microbiota. These interactions can be potential targets for manipulation to provide novel pest management approaches. Frontiers Media S.A. 2017-04-26 /pmc/articles/PMC5405146/ /pubmed/28491055 http://dx.doi.org/10.3389/fmicb.2017.00663 Text en Copyright © 2017 Xia, Gurr, Vasseur, Zheng, Zhong, Qin, Lin, Wang, Song, Li, Lin and You. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xia, Xiaofeng
Gurr, Geoff M.
Vasseur, Liette
Zheng, Dandan
Zhong, Huanzi
Qin, Bingcai
Lin, Junhan
Wang, Yue
Song, FengQin
Li, Yong
Lin, Hailan
You, Minsheng
Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory
title Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory
title_full Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory
title_fullStr Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory
title_full_unstemmed Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory
title_short Metagenomic Sequencing of Diamondback Moth Gut Microbiome Unveils Key Holobiont Adaptations for Herbivory
title_sort metagenomic sequencing of diamondback moth gut microbiome unveils key holobiont adaptations for herbivory
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405146/
https://www.ncbi.nlm.nih.gov/pubmed/28491055
http://dx.doi.org/10.3389/fmicb.2017.00663
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