Cargando…

Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina

Two-pore domain (K2P) potassium channels perform essential roles in neuronal function. These channels produce background leak type potassium currents that act to regulate resting membrane potential and levels of cellular excitability. 15 different K2P channels have been identified in mammals and the...

Descripción completa

Detalles Bibliográficos
Autores principales: Hughes, Steven, Foster, Russell G., Peirson, Stuart N., Hankins, Mark W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405414/
https://www.ncbi.nlm.nih.gov/pubmed/28443635
http://dx.doi.org/10.1038/srep46085
_version_ 1783231762942394368
author Hughes, Steven
Foster, Russell G.
Peirson, Stuart N.
Hankins, Mark W.
author_facet Hughes, Steven
Foster, Russell G.
Peirson, Stuart N.
Hankins, Mark W.
author_sort Hughes, Steven
collection PubMed
description Two-pore domain (K2P) potassium channels perform essential roles in neuronal function. These channels produce background leak type potassium currents that act to regulate resting membrane potential and levels of cellular excitability. 15 different K2P channels have been identified in mammals and these channels perform important roles in a wide number of physiological systems. However, to date there is only limited data available concerning the expression and role of K2P channels in the retina. In this study we conduct the first comprehensive study of K2P channel expression in the retina. Our data show that K2P channels are widely expressed in the mouse retina, with variations in expression detected at different times of day and throughout postnatal development. The highest levels of K2P channel expression are observed for Müller cells (TWIK-1, TASK-3, TRAAK, and TREK-2) and retinal ganglion cells (TASK-1, TREK-1, TWIK-1, TWIK-2 and TWIK-3). These data offer new insight into the channels that regulate the resting membrane potential and electrical activity of retinal cells, and suggests that K2P channels are well placed to act as central regulators of visual signalling pathways. The prominent role of K2P channels in neuroprotection offers novel avenues of research into the treatment of common retinal diseases.
format Online
Article
Text
id pubmed-5405414
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-54054142017-04-27 Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina Hughes, Steven Foster, Russell G. Peirson, Stuart N. Hankins, Mark W. Sci Rep Article Two-pore domain (K2P) potassium channels perform essential roles in neuronal function. These channels produce background leak type potassium currents that act to regulate resting membrane potential and levels of cellular excitability. 15 different K2P channels have been identified in mammals and these channels perform important roles in a wide number of physiological systems. However, to date there is only limited data available concerning the expression and role of K2P channels in the retina. In this study we conduct the first comprehensive study of K2P channel expression in the retina. Our data show that K2P channels are widely expressed in the mouse retina, with variations in expression detected at different times of day and throughout postnatal development. The highest levels of K2P channel expression are observed for Müller cells (TWIK-1, TASK-3, TRAAK, and TREK-2) and retinal ganglion cells (TASK-1, TREK-1, TWIK-1, TWIK-2 and TWIK-3). These data offer new insight into the channels that regulate the resting membrane potential and electrical activity of retinal cells, and suggests that K2P channels are well placed to act as central regulators of visual signalling pathways. The prominent role of K2P channels in neuroprotection offers novel avenues of research into the treatment of common retinal diseases. Nature Publishing Group 2017-04-26 /pmc/articles/PMC5405414/ /pubmed/28443635 http://dx.doi.org/10.1038/srep46085 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hughes, Steven
Foster, Russell G.
Peirson, Stuart N.
Hankins, Mark W.
Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina
title Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina
title_full Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina
title_fullStr Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina
title_full_unstemmed Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina
title_short Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina
title_sort expression and localisation of two-pore domain (k2p) background leak potassium ion channels in the mouse retina
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5405414/
https://www.ncbi.nlm.nih.gov/pubmed/28443635
http://dx.doi.org/10.1038/srep46085
work_keys_str_mv AT hughessteven expressionandlocalisationoftwoporedomaink2pbackgroundleakpotassiumionchannelsinthemouseretina
AT fosterrussellg expressionandlocalisationoftwoporedomaink2pbackgroundleakpotassiumionchannelsinthemouseretina
AT peirsonstuartn expressionandlocalisationoftwoporedomaink2pbackgroundleakpotassiumionchannelsinthemouseretina
AT hankinsmarkw expressionandlocalisationoftwoporedomaink2pbackgroundleakpotassiumionchannelsinthemouseretina